Anti-Ago1 antibody [OTI4G1] (ab279392)
![Anti-Ago1 antibody [OTI4G1] (ab279392)](https://www.abcam.com/ps/products/279/ab279392/Images/ab279392-1-AntiAgo1-antibody-OTI4G1-westerblot-K562-cells-Human-brain.jpg "Anti-Ago1 antibody [OTI4G1] (ab279392)")
Key features and details
- Mouse monoclonal [OTI4G1] to Ago1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Ago1 antibody [OTI4G1]
See all Ago1 primary antibodies -
Description
Mouse monoclonal [OTI4G1] to Ago1 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Ago1 aa 226-490. NP_036331
Database link: Q9UL18 -
Positive control
- IHC-P: Human placenta tissue. Human embryonic cerebellum tissue. WB: K562 cell lysate. Human brain tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
OTI4G1 -
Isotype
IgG1 -
Research areas
Images
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Western blot - Anti-Ago1 antibody [OTI4G1] (ab279392)All lanes : Anti-Ago1 antibody [OTI4G1] (ab279392) at 1/500 dilution
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : Human brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 97 kDa
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ago1 antibody [OTI4G1] (ab279392)](https://www.abcam.com/ps/products/279/ab279392/Images/ab279392-3-AntiAgo1-antibody-OTI4G1-immunohistochemistry-embryonic-cerebellum-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ago1 antibody [OTI4G1] (ab279392)Paraffin embedded human embryonic cerebellum tissue stained for AGO1 using ab279392 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer, pH 8.5, at 120°C for 3 minutes.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ago1 antibody [OTI4G1] (ab279392)](https://www.abcam.com/ps/products/279/ab279392/Images/ab279392-451210-AntiAgo1antibodyOTI4G1immunohistochemistryplacentahuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ago1 antibody [OTI4G1] (ab279392)Paraffin embedded human placenta tissue stained for AGO1 using ab279392 at 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer, pH8.5, at 120°C for 3 minutes.