Anti-Adropin antibody [EPR24382-57] (ab279716)
![Anti-Adropin antibody [EPR24382-57] (ab279716)](https://www.abcam.com/ps/products/279/ab279716/Images/ab279716-451591-anti-adropinantibody-epr2438257-western-blot-protein.jpg "Anti-Adropin antibody [EPR24382-57] (ab279716)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24382-57] to Adropin
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Adropin antibody [EPR24382-57]
See all Adropin primary antibodies -
Description
Rabbit monoclonal [EPR24382-57] to Adropin -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseRatWB MouseRatHuman
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse brain and cerebellum; Rat brain; Human cerebellum tissue lysates; Human Adropin Recombinant Protein (aa38-79) with a His6-ABP tag. IP: Mouse brain; Rat brain tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24382-57 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Adropin antibody (ab279716)Anti-Adropin antibody [EPR24382-57] (ab279716) at 1/1000 dilution + Human Adropin Recombinant Protein (aa38-79) with a His6-ABP tag at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 8 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 8 seconds
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Western blot - Anti-Adropin antibody (ab279716)Anti-Adropin antibody [EPR24382-57] (ab279716) at 1/1000 dilution + Human cerebellum tissue lysate at 20 µg
Secondary
VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))
Predicted band size: 8 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
This blot was developed using a higher sensitivity ECL substrate.
Exposure time: 3 minutes
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Western blot - Anti-Adropin antibody (ab279716)All lanes : Anti-Adropin antibody [EPR24382-57] (ab279716) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse cerebellum tissue lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Mouse lung tissue lysate
Lane 5 : Rat brain tissue lysate
Lane 6 : Rat lung tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 8 kDa
Observed band size: 15,17 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Low expression tissues: lung, kidney (PMID:25074942).
The expression profile / molecular weight observed is consistent with what has been described in the literature (PMID:25074942).
Exposure time: 3 minutes
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Immunoprecipitation - Anti-Adropin antibody (ab279716)Adropin was immunoprecipitated from 0.35 mg Mouse brain tissue lysate with ab279716 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab279716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse brain tissue lysate 10 ug
Lane 2: ab279716 IP in Mouse brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab279716 in mouse brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 6 seconds
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Immunoprecipitation - Anti-Adropin antibody (ab279716)Adropin was immunoprecipitated from 0.35 mg Rat brain tissue lysate with ab279716 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab279716 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat brain tissue lysate 10 ug
Lane 2: ab279716 IP in Rat brain tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab279716 in rat brain tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds