Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19353-16] to Actin - BSA and Azide free
  • Suitable for: IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Actin antibody [EPR19353-16] - BSA and Azide free
    See all Actin primary antibodies

  • Description

    Rabbit monoclonal [EPR19353-16] to Actin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This is a pan-actin antibody. It will detect all 6 actin isoforms corresponding to SwissProt IDs P63261, P60709, P68032, P63267, P62736 and P68133.

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251552 is the carrier-free version of ab216070. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251552 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
    Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
    All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution

    Lane 1 : Human Gamma actin (ACTG1) recombinant protein at 0.01 µg
    Lane 2 : Human Beta actin (ACTB) recombinant protein at 0.02 µg
    Lane 3 : Human Gamma-2 actin (ACTG2) recombinant protein at 0.02 µg
    Lane 4 : Human Alpha-cardiac actin (ACTC) recombinant protein at 0.02 µg
    Lane 5 : Human Alpha-actin-2 (ACTA2) recombinant protein at 0.01 µg
    Lane 6 : Human Alpha-actin-1 (ACTA1) recombinant protein at 0.01 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa


    Exposure time: 1 minute


    This data was developed using ab216070, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human Gamma actin (ACTG1) contains aa2-182 with His-tag. Human Beta actin (ACTB) contains aa1-100 with GST and His tag. Human Gamma-2 actin (ACTG2) contains aa1-200 with His-tag. Human Alpha-cardiac actin (ACTC) contains aa1-200 with His-tag. Human Alpha-actin-2 (ACTA2) contains aa3-253 with His-tag. Human Alpha-actin-1 (ACTA1) contains aa3-200 with His-tag.

  • Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
    Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
    All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
    Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    This data was developed using ab216070, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
    Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
    All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Human fetal kidney tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 30 seconds


    This data was developed using ab216070, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
    Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
    All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : Mouse brain lysate
    Lane 5 : Mouse spleen lysate
    Lane 6 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 3 minutes


    This data was developed using ab216070, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
    Immunoprecipitation - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
    This data was developed using ab216070, the same antibody clone in a different buffer formulation.Actin was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab216070 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216070 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution Lane 1: NIH/3T3 whole cell lysate, 10 μg (Input). Lane 2: ab216070 IP in NIH/3T3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216070 in NIH/3T3 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes. Note: Customer optimization may be required.
  • Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
    Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)

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