Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
![Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)](https://www.abcam.com/ps/products/251/ab251552/Images/ab251552-410599-anti-actin-antibody-epr19353-16-bsa-and-azide-free-western-blot.jpg "Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19353-16] to Actin - BSA and Azide free
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Actin antibody [EPR19353-16] - BSA and Azide free
See all Actin primary antibodies -
Description
Rabbit monoclonal [EPR19353-16] to Actin - BSA and Azide free -
Host species
Rabbit -
Specificity
This is a pan-actin antibody. It will detect all 6 actin isoforms corresponding to SwissProt IDs P63261, P60709, P68032, P63267, P62736 and P68133.
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Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251552 is the carrier-free version of ab216070. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251552 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Clonality
Monoclonal -
Clone number
EPR19353-16 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution
Lane 1 : Human Gamma actin (ACTG1) recombinant protein at 0.01 µg
Lane 2 : Human Beta actin (ACTB) recombinant protein at 0.02 µg
Lane 3 : Human Gamma-2 actin (ACTG2) recombinant protein at 0.02 µg
Lane 4 : Human Alpha-cardiac actin (ACTC) recombinant protein at 0.02 µg
Lane 5 : Human Alpha-actin-2 (ACTA2) recombinant protein at 0.01 µg
Lane 6 : Human Alpha-actin-1 (ACTA1) recombinant protein at 0.01 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Exposure time: 1 minuteThis data was developed using ab216070, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human Gamma actin (ACTG1) contains aa2-182 with His-tag. Human Beta actin (ACTB) contains aa1-100 with GST and His tag. Human Gamma-2 actin (ACTG2) contains aa1-200 with His-tag. Human Alpha-cardiac actin (ACTC) contains aa1-200 with His-tag. Human Alpha-actin-2 (ACTA2) contains aa3-253 with His-tag. Human Alpha-actin-1 (ACTA1) contains aa3-200 with His-tag.
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![Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)](https://www.abcam.com/ps/products/251/ab251552/Images/ab251552-408328-anti-actin-antibody-epr19353-16-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution
Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesThis data was developed using ab216070, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)](https://www.abcam.com/ps/products/251/ab251552/Images/ab251552-406249-anti-actin-antibody-epr19353-16-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 secondsThis data was developed using ab216070, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)](https://www.abcam.com/ps/products/251/ab251552/Images/ab251552-403429-anti-actin-antibody-epr19353-16-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Rat spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutesThis data was developed using ab216070, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Immunoprecipitation - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)](https://www.abcam.com/ps/products/251/ab251552/Images/ab251552-1-anti-actin-antibody-epr19353-16-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)This data was developed using ab216070, the same antibody clone in a different buffer formulation.Actin was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab216070 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216070 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution Lane 1: NIH/3T3 whole cell lysate, 10 μg (Input). Lane 2: ab216070 IP in NIH/3T3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216070 in NIH/3T3 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 3 minutes. Note: Customer optimization may be required. -
![Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)](https://www.abcam.com/ps/products/251/ab251552/Images/ab251552-6-benefits-of-recombinant-antibodies.png)
Anti-Actin antibody [EPR19353-16] - BSA and Azide free (ab251552)
