Antibodies, Proteins, Kits and Reagents for Life Science

298 185 ₸ Product size

100 µl 298 185 ₸

Order now and get it on Tuesday March 02, 2021

Anti-CAPZB antibody [EPR10236] (ab175212)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10236] to CAPZB
Suitable for: IHC-P, WB, Flow Cyt, IP
Reacts with: Human

Product name

Anti-CAPZB antibody [EPR10236]
See all CAPZB primary antibodies
Description

Rabbit monoclonal [EPR10236] to CAPZB
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human CAPZB aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P47756
Positive control
- HeLa, 293T, Jurkat and A431 cell lysates; Human kidney and placenta tissues; Jurkat cells
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10236
Isotype

IgG
Research areas

Western blot - Anti-CAPZB antibody [EPR10236] (ab175212)

All lanes : Anti-CAPZB antibody [EPR10236] (ab175212) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 31 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZB antibody [EPR10236] (ab175212)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CAPZB with ab175212 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZB antibody [EPR10236] (ab175212)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CAPZB with ab175212 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-CAPZB antibody [EPR10236] (ab175212)

Flow cytometric analysis of permeabilized Jurkat cells labeling CAPZB with ab175212 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).
Immunoprecipitation - Anti-CAPZB antibody [EPR10236] (ab175212)

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using ab175212.
Anti-CAPZB antibody [EPR10236] (ab175212)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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