Anti-CAPZB antibody [EPR10236] (ab175212)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10236] to CAPZB
- Suitable for: IHC-P, WB, Flow Cyt, IP
- Reacts with: Human
Overview
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Product name
Anti-CAPZB antibody [EPR10236]
See all CAPZB primary antibodies -
Description
Rabbit monoclonal [EPR10236] to CAPZB -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanIHC-P HumanIP HumanWB Human -
Immunogen
Synthetic peptide within Human CAPZB aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P47756 -
Positive control
- HeLa, 293T, Jurkat and A431 cell lysates; Human kidney and placenta tissues; Jurkat cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10236 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-CAPZB antibody [EPR10236] (ab175212) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 31 kDa
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CAPZB with ab175212 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CAPZB with ab175212 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow cytometric analysis of permeabilized Jurkat cells labeling CAPZB with ab175212 at 1/10 dilution (red) compared to a Rabbit IgG negative control (green).
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Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate using ab175212.
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