Antibodies, Proteins, Kits and Reagents for Life Science

278 083 ₸ Product size

100 µl 278 083 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Actin antibody [EPR19353-16] (ab216070)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19353-16] to Actin
Suitable for: IP, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-Actin antibody [EPR19353-16]
See all Actin primary antibodies
Description

Rabbit monoclonal [EPR19353-16] to Actin
Host species

Rabbit
Specificity

This is a pan-actin antibody. It will detect all 6 actin isoforms corresponding to SwissProt IDs P63261, P60709, P68032, P63267, P62736 and P68133.
Tested Applications & Species

Application Species
IP
Mouse

WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human Gamma actin (ACTG1), beta actin (ACTB), gamma 2 actin (ACTG2), Alpha-cardiac actin (ACTC), Alpha-actin-2 (ACTA2) and Alpha-actin-1 (ACTA1) recombinant proteins. Jurkat, HepG2, A549, HeLa, C6, PC-12 and NIH/3T3 whole cell lysates. Human skeletal muscle and fetal kidney lysates; mouse brain and spleen lysates, and rat brain lysate. IP: NIH/3T3 whole cell lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Mouse
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19353-16
Isotype

IgG
Research areas

Western blot - Anti-Actin antibody [EPR19353-16] (ab216070)

All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution

Lane 1 : Human Gamma actin (ACTG1) recombinant protein at 0.01 µg
Lane 2 : Human Beta actin (ACTB) recombinant protein at 0.02 µg
Lane 3 : Human Gamma-2 actin (ACTG2) recombinant protein at 0.02 µg
Lane 4 : Human Alpha-cardiac actin (ACTC) recombinant protein at 0.02 µg
Lane 5 : Human Alpha-actin-2 (ACTA2) recombinant protein at 0.01 µg
Lane 6 : Human Alpha-actin-1 (ACTA1) recombinant protein at 0.01 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 42 kDa

Exposure time: 1 minute

Blocking/Dilution buffer: 5% NFDM/TBST.

Human Gamma actin (ACTG1) contains aa2-182 with His-tag. Human Beta actin (ACTB) contains aa1-100 with GST and His tag. Human Gamma-2 actin (ACTG2) contains aa1-200 with His-tag. Human Alpha-cardiac actin (ACTC) contains aa1-200 with His-tag. Human Alpha-actin-2 (ACTA2) contains aa3-253 with His-tag. Human Alpha-actin-1 (ACTA1) contains aa3-200 with His-tag.
Western blot - Anti-Actin antibody [EPR19353-16] (ab216070)

All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution

Lane 1 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 10 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Actin antibody [EPR19353-16] (ab216070)

All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution

Lane 1 : Human skeletal muscle lysate
Lane 2 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Actin antibody [EPR19353-16] (ab216070)

All lanes : Anti-Actin antibody [EPR19353-16] (ab216070) at 1/1000 dilution

Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
Lane 4 : Mouse brain lysate
Lane 5 : Mouse spleen lysate
Lane 6 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 42 kDa
Observed band size: 42 kDa

Exposure time: 3 minutes

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-Actin antibody [EPR19353-16] (ab216070)

Actin was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab216070 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab216070 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution

Lane 1: NIH/3T3 whole cell lysate, 10 μg (Input).

Lane 2: ab216070 IP in NIH/3T3 whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab216070 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.

Note: Customer optimization may be required.
Anti-Actin antibody [EPR19353-16] (ab216070)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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