Anti-ACRBP antibody (ab64809)
Key features and details
- Rabbit polyclonal to ACRBP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACRBP antibody -
Description
Rabbit polyclonal to ACRBP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
A synthetic peptide derived from an internal region of human ACRBP.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACRBP antibody (ab64809)IHC image of ab64809 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64809, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Western blot - Anti-ACRBP antibody (ab64809)All lanes : Anti-ACRBP antibody (ab64809) at 1/500 dilution
Lane 1 : Extracts of 293 cells
Lane 2 : Extracts of HepG2 cells
Lane 3 : Extracts of Jurkat cells
Lane 4 : Extracts of Jurkat cells with immunizing Peptide
Lysates/proteins at 5 µg per lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa