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Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8282(B)] to Aconitase 2 - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free
    See all Aconitase 2 primary antibodies

  • Description

    Rabbit monoclonal [EPR8282(B)] to Aconitase 2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab248277 is the carrier-free version of ab129069. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab248277 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)
    Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)

    This data was developed using ab129069, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)

    Lane 2: Aconitase 2 knockout HAP1 whole cell lysate (20 µg)

    Lane 3: HeLa whole cell lysate (20 µg)

    Lane 4: Jurkat whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab129069 observed at 90 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab129069 was shown to specifically react with Aconitase 2 in wild-type HAP1 cells as signal was lost in Aconitase 2 knockout cells. Wild-type and Aconitase 2 knockout samples were subjected to SDS-PAGE. ab129069 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)
    Western blot - Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)
    All lanes : Anti-Aconitase 2 antibody [EPR8282(B)] (ab129069) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Human fetal heart tissue lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP-conjugated at 1/2000 dilution

    Predicted band size: 85 kDa



    This data was developed using ab129069, the same antibody clone in a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)
    This data was developed using ab129069, the same antibody clone in a different buffer formulation.ab129069, at 1/100 dilution, staining Aconitase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)
    OI-RD Scanning - Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)
    This data was developed using ab129069, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)
    Anti-Aconitase 2 antibody [EPR8282(B)] - BSA and Azide free (ab248277)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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