Anti-ACK1 (phospho Y284) antibody (ab74091)
Key features and details
- Rabbit polyclonal to ACK1 (phospho Y284)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ACK1 (phospho Y284) antibody
See all ACK1 primary antibodies -
Description
Rabbit polyclonal to ACK1 (phospho Y284) -
Host species
Rabbit -
Specificity
ab74091 detects ACK1 only when phosphorylated at tyrosine 284. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic phosphopeptide derived from human ACK1 around the phosphorylation site of tyrosine 284 (D-H-YP-V-M).
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Positive control
- Human breast carcinoma tissue, A549 cells, and HepG2 cell extracts treated with EGF (200ng/ml, 30mins).
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab74091 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ACK1 (phospho Y284) antibody (ab74091)All lanes : Anti-ACK1 (phospho Y284) antibody (ab74091) at 1/500 dilution
Lane 1 : HepG2 cell extracts
treated with EGF (200ng/ml, 30mins)
Lane 2 : HepG2 cell extracts
treated with EGF (200ng/ml, 30mins) with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 114 kDa
Observed band size: >117 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-ACK1 (phospho Y284) antibody (ab74091)ab74091 at 1/500 dilution staining ACK1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ACK1 (phospho Y284) antibody (ab74091)ab74091 at 1/50 dilution staining ACK1 in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
