Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24299-52] to ABP1 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free
    See all ABP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR24299-52] to ABP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-P, WBmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human kidney, colon and stomach tissue lysate; THP-1 whole cell lysate. IHC-P: Human placenta and kidney tissue. Flow Cyt (intra): THP-1 cells.

  • General notes

    ab278501 is the carrier-free version of ab278497.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C.

  • Storage buffer

    Constituent: 100% PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR24299-52

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)
    Western blot - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)
    All lanes : Anti-ABP1 antibody [EPR24299-52] (ab278497) at 1/1000 dilution

    Lane 1 : Human kidney tissue lysate
    Lane 2 : Human colon tissue lysate
    Lane 3 : Human stomach tissue lysate
    Lane 4 : Human hypothalamus tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/2000 dilution

    Predicted band size: 85 kDa
    Observed band size: 85 kDa



    This data was developed using ab278497, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    The expression profile observed is consistent with what has been described in the literature (PMID:25037221, 12072962).

    Negative control: human hypothalamus (PMID:12072962).

    Samples are non-boiled as boiling may cause protein aggregates. 

    Exposure time: Lane 1: 15 seconds; Lanes 2-4: 103 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)

    This data was developed using ab278497, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling ABP1 with ab278497 at 1/2000 dilution (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on human placenta (PMID:12072962). The section was incubated with ab278497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)
    Flow Cytometry - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)

    This data was developed using ab278497, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) cells labelling ABP1 with ab278497 at 1/50 dilution (1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)
    Western blot - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)
    Anti-ABP1 antibody [EPR24299-52] (ab278497) at 1/1000 dilution + THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 85 kDa
    Observed band size: 85 kDa



    This data was developed using ab278497, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Sample is non-boiled as boiling may cause protein aggregates.

    Exposure time: 37 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)

    This data was developed using ab278497, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ABP1 with ab278497 at 1/2000 dilution (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Positive staining on human kidney (PMID:12072962). The section was incubated with ab278497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABP1 antibody [EPR24299-52] - BSA and Azide free (ab278501)

    This data was developed using ab278497, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling ABP1 with ab278497 at 1/2000 dilution (0.248 ug/ml) followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used. Negative control: No staining on human cerebrum (PMID:12072962). The section was incubated with ab278497 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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