Anti-ABCA4 antibody [3F4] (ab77285)
![Anti-ABCA4 antibody [3F4] (ab77285)](https://www.abcam.com/ps/products/77/ab77285/Images/ab77285-93012-anti-abca4-antibody-3f4-immunohistochemistry.gif "Anti-ABCA4 antibody [3F4] (ab77285)")
Key features and details
- Mouse monoclonal [3F4] to ABCA4
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ABCA4 antibody [3F4]
See all ABCA4 primary antibodies -
Description
Mouse monoclonal [3F4] to ABCA4 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Full length native protein (purified) corresponding to Cow ABCA4. 220 kDa
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Epitope
Epitope has been mapped to aa 2252 – 2262 bovine ABCA4 protein, see Pubmed 9092582 -
Positive control
- Adult mouse retina tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.50
Constituents: 0.01% BSA, 50% Glycerol, 0.87% Sodium chloride, 0.238% HEPES -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab77285 is protein G purified from culture supernatant. -
Clonality
Monoclonal -
Clone number
3F4 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA4 antibody [3F4] (ab77285)ab77285, at a 1/100 dilution, staining ABCA4 in formalin fixed, paraffin embedded adult mouse retina tissue by Immunohistochemisty.
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![Western blot - Anti-ABCA4 antibody [3F4] (ab77285)](https://www.abcam.com/ps/products/77/ab77285/Images/ab77285-93011-anti-abca4-antibody-3f4-western-blot.jpg)
Western blot - Anti-ABCA4 antibody [3F4] (ab77285)All lanes : Anti-ABCA4 antibody [3F4] (ab77285) at 1/500 dilution
Lane 1 : WERI (Human Retinoblastoma) Whole Cell Lysate
Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 3 : Rat Retina Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 257 kDa
Observed band size: 257 kDa
Additional bands at: 142 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
