Anti-A RAF (phospho S299) antibody (ab112053)
Key features and details
- Rabbit polyclonal to A RAF (phospho S299)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-A RAF (phospho S299) antibody
See all A RAF primary antibodies -
Description
Rabbit polyclonal to A RAF (phospho S299) -
Host species
Rabbit -
Specificity
ab112053 detects endogenous levels of A RAF only when phosphorylated at serine 299. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic phosphopeptide derived from Human A RAF around the phosphorylation site of serine 299 (R-D-SP-G-Y).
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Positive control
- Human colon carcinoma tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab112053 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-A RAF (phospho S299) antibody (ab112053)ab112053 at 1/50 dilution staining A RAF in Paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry. The image on the right is treated with the synthesized phospho peptide.
