Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 90 460 ₸

Order now and get it on Wednesday March 10, 2021

Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [NfL23] to 68kDa Neurofilament/NF-L
Suitable for: WB, IHC-P
Reacts with: Mouse, Rat, Human

Product name

Anti-68kDa Neurofilament/NF-L antibody [NfL23]
See all 68kDa Neurofilament/NF-L primary antibodies
Description

Mouse monoclonal [NfL23] to 68kDa Neurofilament/NF-L
Host species

Mouse

Tested Applications & Species

Application	Species
IHC-P	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human, mouse and rat cerebrum tissue. WB: SK-N-BE(2) whole cell lysate. Human cerebrospinal fluid. Mouse hippocampus tissue lysate. Rat hippocampus and brain tissue lysate.
General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

NfL23
Isotype

IgG1
Research areas

Western blot - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

All lanes : Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442) at 1/1000 dilution

Lane 1 : SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate at 20 µg
Lane 2 : Human cerebrospinal fluid at 40 µl

Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

Predicted band size: 62 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

Exposure times: Lane 1: 37 seconds; Lane 2: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurofilament light with ab273442 at 1/500 (1.986 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab273442 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Western blot - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

All lanes : Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442) at 1/1000 dilution

Lane 1 : Mouse hippocampus tissue lysate
Lane 2 : Mouse liver tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat hippocampus tissue lysate
Lane 5 : Rat liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

Predicted band size: 62 kDa
Observed band size: 68 kDa
why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofilament light with ab273442 at 1/500 (1.986 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on mouse cerebrum is observed. The section was incubated with ab273442 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofilament light with ab273442 at 1/500 (1.986 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection). Positive staining on rat cerebrum is observed. The section was incubated with ab273442 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

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