Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [NfL23] to 68kDa Neurofilament/NF-L
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-68kDa Neurofilament/NF-L antibody [NfL23]
    See all 68kDa Neurofilament/NF-L primary antibodies

  • Description

    Mouse monoclonal [NfL23] to 68kDa Neurofilament/NF-L

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human, mouse and rat cerebrum tissue. WB: SK-N-BE(2) whole cell lysate. Human cerebrospinal fluid. Mouse hippocampus tissue lysate. Rat hippocampus and brain tissue lysate.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    NfL23

  • Isotype

    IgG1

  • Research areas

Images

  • Western blot - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)
    Western blot - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)
    All lanes : Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442) at 1/1000 dilution

    Lane 1 : SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate at 20 µg
    Lane 2 : Human cerebrospinal fluid at 40 µl

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 62 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure times: Lane 1: 37 seconds; Lane 2: 3 minutes.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Neurofilament light with ab273442 at 1/500 (1.986 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on human cerebrum is observed. The section was incubated with ab273442 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)
    Western blot - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)
    All lanes : Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442) at 1/1000 dilution

    Lane 1 : Mouse hippocampus tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat hippocampus tissue lysate
    Lane 5 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-mouse IgG for IP (HRP) (ab131368) at 1/1000 dilution

    Predicted band size: 62 kDa
    Observed band size: 68 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Neurofilament light with ab273442 at 1/500 (1.986 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on mouse cerebrum is observed. The section was incubated with ab273442 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Neurofilament light with ab273442 at 1/500 (1.986 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond® Polymer Refine Detection). Positive staining on rat cerebrum is observed. The section was incubated with ab273442 for 30 mins at room temperature and blocked mouse IgG with specific antibody ab125913 for 8min. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond® Polymer Refine Detection) was used.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)
    Anti-68kDa Neurofilament/NF-L antibody [NfL23] (ab273442)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.