Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-2B4 antibody [EPR23692-33] (ab256370)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23692-33] to 2B4
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-2B4 antibody [EPR23692-33]
    See all 2B4 primary antibodies

  • Description

    Rabbit monoclonal [EPR23692-33] to 2B4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: NK-92, KG-1a and THP-1 whole cell lysates; Human spleen tissue lysate. IHC-P: Human tonsil and non-Hodgkin's lymphoma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-2B4 antibody [EPR23692-33] (ab256370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-2B4 antibody [EPR23692-33] (ab256370)

    Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma tissue labeling 2B4 with ab256370 at 1/50 (12.04 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human non-Hodgkin's lymphoma (PMID: 19499526). The section was incubated with ab256370 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-2B4 antibody [EPR23692-33] (ab256370)
    Western blot - Anti-2B4 antibody [EPR23692-33] (ab256370)
    All lanes : Anti-2B4 antibody [EPR23692-33] (ab256370) at 1/1000 dilution

    Lane 1 : NK-92 (human malignant non-Hodgkins lymphoma natural killer cell) whole cell lysate
    Lane 2 : NK-92 whole cell lysate treated with PNGase F
    Lane 3 : A549 (human lung carcinoma epithelial cell) whole cell lysate
    Lane 4 : THP-1 (human monocytic leukemia monocyte) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 37,42,70-100 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 19499526, 11099315, 16177062, 28126968).

    Negative control: A549 (PMID: 23192659 and Human Protein Atlas).

    Exposure time: Lanes 1-2: 37 seconds; Lanes 3-4: 125 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-2B4 antibody [EPR23692-33] (ab256370)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-2B4 antibody [EPR23692-33] (ab256370)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling 2B4 with ab256370 at 1/50 (12.04 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human tonsil (PMID:19499526). The section was incubated with ab256370 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-2B4 antibody [EPR23692-33] (ab256370)
    Western blot - Anti-2B4 antibody [EPR23692-33] (ab256370)
    Anti-2B4 antibody [EPR23692-33] (ab256370) at 1/1000 dilution + Human spleen tissue lysate at 20 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 48 seconds.

     

  • Western blot - Anti-2B4 antibody [EPR23692-33] (ab256370)
    Western blot - Anti-2B4 antibody [EPR23692-33] (ab256370)
    Anti-2B4 antibody [EPR23692-33] (ab256370) at 1/1000 dilution + KG-1a (human bone marrow myeloblast) whole cell lysate at 10 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/5000 dilution

    Predicted band size: 42 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 24 seconds.

     

  • Anti-2B4 antibody [EPR23692-33] (ab256370)
    Anti-2B4 antibody [EPR23692-33] (ab256370)

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