Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)
![Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)](https://www.abcam.com/ps/products/228/ab228858/Images/ab228858-449851-Anti1methylguanosine-m1G-antibody-EPR19833150-dot-blot-2.jpg "Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19833-150] to 1-methylguanosine (m1G) - BSA and Azide free
- Suitable for: Dot blot, ELISA, IP
- Reacts with: Species independent
Overview
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Product name
Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free
See all 1-methylguanosine (m1G) primary antibodies -
Description
Rabbit monoclonal [EPR19833-150] to 1-methylguanosine (m1G) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, ELISA, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule within 1-methylguanosine (m1G). The exact sequence is proprietary.
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General notes
ab228858 is the carrier-free version of ab208199. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab228858 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19833-150 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)
This data was developed using ab208199, the same antibody clone in a different buffer formulation.
Dot blot analysis of 1-methylguanosine (m1G) using ab208199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution (Upper) or a Streptavidin-Alexa®488 conjugate (Lower).
Lane 1: Biotin-m1G RNA oligo
Lane 2: Biotin-G RNA oligo
Lane 3: Biotin-m2G RNA oligo
Lane 4: Biotin-m22G RNA oligo
Exposure time 5 seconds.
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![Homogeneous plate-based assay - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)](https://www.abcam.com/ps/products/228/ab228858/Images/ab228858-449852-Anti1methylguanosine-m1G-antibody-EPR19833150-elisa-htrf.jpg)
Homogeneous plate-based assay - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)This data was developed using ab208199, the same antibody clone in a different buffer formulation.
HTRF was performed on 25 nM of each biotinylated oligo using ab208199 at a concentration range of 0.001-4.000 µg/ml.
Species-specific Terbium cryptate-labelled anti-rabbit IgG antibody and D2-labeled Streptavidin were used.
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![Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)](https://www.abcam.com/ps/products/228/ab228858/Images/ab228858-449850-Anti1methylguanosine-m1G-antibody-EPR19833150-dot-blot-1.jpg)
Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)This data was developed using ab208199, the same antibody clone in a different buffer formulation.
Dot blot analysis of 1-methylguanosine (m1G) using ab208199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution (Left) or Methylene Blue (Right).
Lane 1: HeLa total RNA
Lane 2: Yeast tRNA
Exposure time: 8 seconds
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![Immunoprecipitation - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)](https://www.abcam.com/ps/products/228/ab228858/Images/ab228858-294204-m1G.jpg)
Immunoprecipitation - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)This data was developed using ab208199, the same antibody clone in a different buffer formulation.
The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.
ab208199 (0.2 μg) was coated into Dynabeads® sheep-anti-rabbit IgG (50 μl) for 1h at RT.
Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.
After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.
ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.
Lane 1: Buffer only.
Lane 2: Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[m1G]*.mN.mN.mN.mN.mN 3’
Lane 3: Unmodified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[G]*.mN.mN.mN.mN.mN 3’
N - equimolar mixture of (A/U/G/C)
m - 2’O methyl protection
* - phosphorothioate protectionBlocking buffer and concentration: 5% NFDM/TBST
Dilution buffer and concentration: TBST/0.1% Triton X-100/1 mM EDTA
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![ELISA - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)](https://www.abcam.com/ps/products/228/ab228858/Images/ab228858-450930-Anti1methylguanosine-m1G-antibody-EPR19833150-elisa.jpg)
ELISA - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] - BSA and Azide free (ab228858)This data was developed using ab208199, the same antibody clone in a different buffer formulation.
BSA-conjugated m1G (modified) and G (unmodified) nucleosides were coated onto wells of a 96 well plate. ELISA was performed on 1.0 µg/ml of antigen using ab208199 at a concentration range of 0.005-4.000 µg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.