Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)
![Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)](https://www.abcam.com/ps/products/251/ab251526/Images/ab251526-4-anti-n2-n2-dimethylguanosine-m2-2g-antibody-epr-19838-40-bsa-and-azide-free-dot-blot-biotin-m22g-oligo-m22g-m2g-m1g.jpg "Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR-19838-40] to N2,N2-dimethylguanosine (m2,2G) - BSA and Azide free
- Suitable for: ELISA, IP, Dot blot
Overview
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Product name
Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free
See all N2,N2-dimethylguanosine (m2,2G) primary antibodies -
Description
Rabbit monoclonal [EPR-19838-40] to N2,N2-dimethylguanosine (m2,2G) - BSA and Azide free -
Host species
Rabbit -
Specificity
Has been developed to discriminate between the modified base N2,N2-dimethylguanosine (m2,2G) and the unmodified counterpart Guanosine (G).
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Tested applications
Suitable for: ELISA, IP, Dot blotmore details -
Species reactivity
Reacts with: Synthetic fragment -
Immunogen
Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab251526 is the carrier-free version of ab211488. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab251526 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR-19838-40 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)This data was developed using ab211488, the same antibody clone in a different buffer formulation.Primary antibody dilution: 1/500 Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Secondary antibody dilution: 1/100,000 Blocking and dilution buffer: AdvanBlockTM Chemi Blocking buffer Input: Biotin-m2.2G oligo 2 ng per Dot Competitive nucleosides: m2.2G, m2G, m1G Exposure times: 37 seconds
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![Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)](https://www.abcam.com/ps/products/251/ab251526/Images/ab251526-3-anti-n2-n2-dimethylguanosine-m2-2g-antibody-epr-19838-40-bsa-and-azide-free-dot-blot-yeast-rna-biotin-m22g-rna-oligo-biotin-g-rna-oligo.jpg)
Dot Blot - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)This data was developed using ab211488, the same antibody clone in a different buffer formulation.Lane 1: Yeast tRNA Lane 2: Biotin-m2.2G RNA oligo Lane 3: Biotin-G RNA oligo Primary antibody dilution: 1/500 Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated Secondary antibody dilution: 1/20,000 Blocking and dilution buffer: AdvanBlockTM Chemi Blocking buffer -
![Immunoprecipitation - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)](https://www.abcam.com/ps/products/251/ab251526/Images/ab251526-403549-anti-n2-n2-dimethylguanosine-m2-2g-antibody-epr-19838-40-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)This data was developed using ab211488, the same antibody clone in a different buffer formulation.
The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.
ab211488 (0.2 µg) was coated into Dynabeads® sheep-anti-rabbit IgG (50 µl) for 1h at RT.
Unmodified/modified oligonucleotides (5 µM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.
After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.
ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.
Lane 1: Buffer only.
Lane 2: Modified oligonucleotide (5 µM), 5' Biotin-mN.mN.mN.mN.mN.[m2,2G]*.mN.mN.mN.mN.mN 3'
Lane 3: Unmodified oligonucleotide (5 µM), 5' Biotin-mN.mN.mN.mN.mN.[G]*.mN.mN.mN.mN.mN 3'
N - equimolar mixture of (A/U/G/C)
m - 2'O methyl protection
* - phosphorothioate protectionBlocking buffer: 5% NFDM/TBST
Dilution buffer: TBST/0.1% Triton X-100/1 mM EDTA.
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![ELISA - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)](https://www.abcam.com/ps/products/251/ab251526/Images/ab251526-1-anti-n2-n2-dimethylguanosine-m2-2g-antibody-epr-19838-40-bsa-and-azide-free-elisa.jpg)
ELISA - Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)This data was developed using ab211488, the same antibody clone in a different buffer formulation.BSA-conjugated m2,2G (modified) and G (unmodified) nucleosides were coated onto wells of a 96 well plate. ELISA was performed on 1.0 µg/ml of antigen using ab211488 at a concentration range of 0.005-4.000 µg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution. -
![Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)](https://www.abcam.com/ps/products/251/ab251526/Images/ab251526-5-benefits-of-recombinant-antibodies.png)
Anti-N2,N2-dimethylguanosine (m2,2G) antibody [EPR-19838-40] - BSA and Azide free (ab251526)