Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (ab211494)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR -19850-108] to N4-methylcytidine (m4C)
  • Suitable for: IP, ELISA

Overview

  • Product name

    Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108]
    See all N4-methylcytidine (m4C) primary antibodies

  • Description

    Rabbit monoclonal [EPR -19850-108] to N4-methylcytidine (m4C)

  • Host species

    Rabbit

  • Specificity

    Has been developed to discriminate between the modified base N4-methylcytidine (m4C) and the unmodified counterpart Cytidine (C).

  • Tested applications

    Suitable for: IP, ELISAmore details

  • Species reactivity

    Reacts with: Synthetic fragment

  • Immunogen

    Chemical/ Small Molecule. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: 5' Biotin-mN.mN.mN.mN.mN.[m4C]*.mN.mN.mN.mN.mN 3' * - phosphorothioate protection. ELISA: Biotinylated m4C-modified oligonucleotides.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR -19850-108

  • Isotype

    IgG

  • Research areas

Images

  • Immunoprecipitation - Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (ab211494)
    Immunoprecipitation - Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (ab211494)

    The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.

    ab211494 (0.2 μg) was coated into Dynabeads® sheep-anti-rabbit IgG (50 μl) for 1h at RT.

    Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.

    After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.

    ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.

    Lane 1: Buffer only.

    Lane 2: Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[m4C]*.mN.mN.mN.mN.mN 3’

    Lane 3: Unmodified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[C]*.mN.mN.mN.mN.mN 3’

    N - equimolar mixture of (A/U/G/C)
    m - 2’O methyl protection
    * - phosphorothioate protection 

    Blocking buffer and concentration: 5% NFDM/TBST

    Dilution buffer and concentration: TBST/0.1% Triton X-100/1 mM EDTA

  • ELISA - Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (ab211494)
    ELISA - Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (ab211494)

    Biotinylated m4C (modified) and C (unmodified) oligonucleotides with the below sequence were coated onto wells of a 96 well plate. 

    Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[m4C]*.mN.mN.mN.mN.mN 3’

    Unmodified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[C]*.mN.mN.mN.mN.mN 3’

    N - equimolar mixture of (A/U/G/C)
    m - 2’O methyl protection
    * - phosphorothioate protection

    ELISA was performed on 1.0 µg/ml of antigen using ab211494 at a concentration range of 0.005-4.000 µg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.

  • Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (ab211494)
    Anti-N4-methylcytidine (m4C) antibody [EPR -19850-108] (ab211494)

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