Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)
![Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)](https://www.abcam.com/ps/products/208/ab208199/Images/ab208199-449848-Anti1methylguanosine-m1G-antibody-EPR19833150-dot-blot-2.jpg "Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19833-150] to 1-methylguanosine (m1G)
- Suitable for: Dot blot, ELISA, IP
- Reacts with: Species independent
Overview
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Product name
Anti-1-methylguanosine (m1G) antibody [EPR19833-150]
See all 1-methylguanosine (m1G) primary antibodies -
Description
Rabbit monoclonal [EPR19833-150] to 1-methylguanosine (m1G) -
Host species
Rabbit -
Tested applications
Suitable for: Dot blot, ELISA, IPmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule within 1-methylguanosine (m1G). The exact sequence is proprietary.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19833-150 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)
Dot blot analysis of 1-methylguanosine (m1G) using ab208199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution (Upper) or a Streptavidin-Alexa®488 conjugate (Lower).
Lane 1: Biotin-m1G RNA oligo
Lane 2: Biotin-G RNA oligo
Lane 3: Biotin-m2G RNA oligo
Lane 4: Biotin-m22G RNA oligo
Exposure time 5 seconds
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![Homogeneous plate-based assay - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)](https://www.abcam.com/ps/products/208/ab208199/Images/ab208199-449849-Anti1methylguanosine-m1G-antibody-EPR19833150-elisa-htrf.jpg)
Homogeneous plate-based assay - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)HTRF was performed on 25 nM of each biotinylated oligo using ab208199 at a concentration range of 0.001-4.000 µg/ml.
Species-specific Terbium cryptate-labelled anti-rabbit IgG antibody and D2-labeled Streptavidin were used.
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![Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)](https://www.abcam.com/ps/products/208/ab208199/Images/ab208199-449847-Anti1methylguanosine-m1G-antibody-EPR19833150-dot-blot-1.jpg)
Dot Blot - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)Dot blot analysis of 1-methylguanosine (m1G) using ab208199 at 1/500 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20,000 dilution (Left) or Methylene Blue (Right).
Lane 1: HeLa total RNA
Lane 2: Yeast tRNA
Exposure time: 8 seconds
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![Immunoprecipitation - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)](https://www.abcam.com/ps/products/208/ab208199/Images/ab208199-293863-208199-IP.jpg)
Immunoprecipitation - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)The IP was performed in a U-bottom non-adsorbing propylene 96-well plate.
ab208199 (0.2 μg) was coated into Dynabeads® sheep-anti-rabbit IgG (50 μl) for 1h at RT.
Unmodified/modified oligonucleotides (5 μM) were added to samples containing the antibody/bead complexes and incubated with agitation for 1 hour at RT.
After washing, Peroxidase-conjugated Streptavidin was incubated at 1/1000 dilution with agitation for 1 hour at RT.
ECL substrate was then added and the results read in a non-transparent 96-well plate with a digital detector and analyzed using ImageJ.
Lane 1: Buffer only.
Lane 2: Modified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[m1G]*.mN.mN.mN.mN.mN 3’
Lane 3: Unmodified oligonucleotide (5 μM), 5’ Biotin-mN.mN.mN.mN.mN.[G]*.mN.mN.mN.mN.mN 3’
N - equimolar mixture of (A/U/G/C)
m - 2’O methyl protection
* - phosphorothioate protectionBlocking buffer and concentration: 5% NFDM/TBST
Dilution buffer and concentration: TBST/0.1% Triton X-100/1 mM EDTA
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![ELISA - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)](https://www.abcam.com/ps/products/208/ab208199/Images/ab208199-293851-208199-1.jpg)
ELISA - Anti-1-methylguanosine (m1G) antibody [EPR19833-150] (ab208199)BSA-conjugated m1G (modified) and G (unmodified) nucleosides were coated onto wells of a 96 well plate. ELISA was performed on 1.0 µg/ml of antigen using ab208199 at a concentration range of 0.005-4.000 µg/ml, followed by Goat Anti-Rabbit IgG, (H+L), alkaline phosphatase conjugated secondary antibody at 1/2500 dilution.