Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-0N Tau antibody [EPR21726] (ab218199)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21726] to 0N Tau
  • Suitable for: WB, IHC-P, IHC-Fr
  • Reacts with: Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-0N Tau antibody [EPR21726]
    See all 0N Tau primary antibodies

  • Description

    Rabbit monoclonal [EPR21726] to 0N Tau

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Rat
    IHC-P
    Human
    WB
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast carcinoma and cerebrum tissue. IHC-Fr: Rat hippocampus tissue. WB: Recombinant human 0N3R Tau and 0N4R Tau proteins; Human hippocampus and brain lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human cerebrum (PMID: 17183532) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-0N Tau antibody [EPR21726] (ab218199)
    Western blot - Anti-0N Tau antibody [EPR21726] (ab218199)
    All lanes : Anti-0N Tau antibody [EPR21726] (ab218199) at 1/1000 dilution

    Lane 1 : Human hippocampus lysate
    Lane 2 : Human brain lysate
    Lane 3 : Human stomach lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 79 kDa


    Exposure time: 3 minutes


    Negative control: Human stomach PMID:8752131; PMID:11727254.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-0N Tau antibody [EPR21726] (ab218199)
    Western blot - Anti-0N Tau antibody [EPR21726] (ab218199)
    All lanes : Anti-0N Tau antibody [EPR21726] (ab218199) at 1/1000 dilution

    Lane 1 : His-tagged human 2N3R Tau recombinant protein (aa1-410) 10 ng
    Lane 2 : His-tagged human 2N4R Tau recombinant protein (aa1-441) 10ng
    Lane 3 : His-tagged human 0N3R Tau recombinant protein (aa1-352) 10ng
    Lane 4 : His-tagged human 0N4R Tau recombinant protein (aa1-383) 10ng
    Lane 5 : His-tagged human 1N3R Tau recombinant protein (aa1-381) 10ng
    Lane 6 : His-tagged human 1N4R Tau recombinant protein (aa1-412) 10ng

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 79 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    This antibody specifically recognizes 0N3R and 0N4R tau recombinant proteins. The lower weak bands maybe degraded tau fragments (PMID:28045602).

  • Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (ab218199)
    Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus tissue labeling 0N Tau with ab218199 at 1/500 dilution (green), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Cytoplasmic staining on MF (mossy fibers) of rat hippocampus (PMID:18925637, PMID:24386422) is observed. Counterstained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

  • Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (ab218199)
    Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

    Negative control - Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat stomach tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. No staining on rat stomach (PMID:11727254; PMID: 8752131) is observed. Counterstained with DAPI (blue).
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit used at a 1/1,000 dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human breast carcinoma (PMID: 15914550) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

    Negative control - Immunohistochemical analysis of paraffin-embedded human liver tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). No staining on human liver (PMID: 8752131) is observed. Counterstained with hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

  • Anti-0N Tau antibody [EPR21726] (ab218199)
    Anti-0N Tau antibody [EPR21726] (ab218199)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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