Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Tuesday March 02, 2021

Anti-0N Tau antibody [EPR21726] (ab218199)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR21726] to 0N Tau
Suitable for: WB, IHC-P, IHC-Fr
Reacts with: Rat, Human, Recombinant fragment

Product name

Anti-0N Tau antibody [EPR21726]
See all 0N Tau primary antibodies
Description

Rabbit monoclonal [EPR21726] to 0N Tau
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Rat
IHC-P	Human
WB	Human Recombinant fragment

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human breast carcinoma and cerebrum tissue. IHC-Fr: Rat hippocampus tissue. WB: Recombinant human 0N3R Tau and 0N4R Tau proteins; Human hippocampus and brain lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR21726
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human cerebrum (PMID: 17183532) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Western blot - Anti-0N Tau antibody [EPR21726] (ab218199)

All lanes : Anti-0N Tau antibody [EPR21726] (ab218199) at 1/1000 dilution

Lane 1 : Human hippocampus lysate
Lane 2 : Human brain lysate
Lane 3 : Human stomach lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

Predicted band size: 79 kDa

Exposure time: 3 minutes

Negative control: Human stomach PMID:8752131; PMID:11727254.

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-0N Tau antibody [EPR21726] (ab218199)

All lanes : Anti-0N Tau antibody [EPR21726] (ab218199) at 1/1000 dilution

Lane 1 : His-tagged human 2N3R Tau recombinant protein (aa1-410) 10 ng
Lane 2 : His-tagged human 2N4R Tau recombinant protein (aa1-441) 10ng
Lane 3 : His-tagged human 0N3R Tau recombinant protein (aa1-352) 10ng
Lane 4 : His-tagged human 0N4R Tau recombinant protein (aa1-383) 10ng
Lane 5 : His-tagged human 1N3R Tau recombinant protein (aa1-381) 10ng
Lane 6 : His-tagged human 1N4R Tau recombinant protein (aa1-412) 10ng

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

Predicted band size: 79 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.

This antibody specifically recognizes 0N3R and 0N4R tau recombinant proteins. The lower weak bands maybe degraded tau fragments (PMID:28045602).
Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat hippocampus tissue labeling 0N Tau with ab218199 at 1/500 dilution (green), followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. Cytoplasmic staining on MF (mossy fibers) of rat hippocampus (PMID:18925637, PMID:24386422) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1,000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

Negative control - Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat stomach tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at a 1/1,000 dilution. No staining on rat stomach (PMID:11727254; PMID: 8752131) is observed. Counterstained with DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor^®488 Goat anti-Rabbit used at a 1/1,000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on human breast carcinoma (PMID: 15914550) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-0N Tau antibody [EPR21726] (ab218199)

Negative control - Immunohistochemical analysis of paraffin-embedded human liver tissue labeling 0N Tau with ab218199 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). No staining on human liver (PMID: 8752131) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-0N Tau antibody [EPR21726] (ab218199)

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