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Annexin V-iFluor 700 Apoptosis Staining / Detection Reagent (ab219910)

Key features and details

  • Assay type: Quantitative
  • Detection method: Fluorescent
  • Platform: Flow cytometer
  • Sample type: Adherent cells, Suspension cells

Overview

  • Product name

    Annexin V-iFluor 700 Apoptosis Staining / Detection Reagent
    See all Annexin V/ANXA5 kits

  • Detection method

    Fluorescent

  • Sample type

    Adherent cells, Suspension cells

  • Assay type

    Quantitative

  • Species reactivity

    Reacts with: Mammals, Other species

  • Product overview

    Annexin V-iFluor 700 Apoptosis Staining / Detection Reagent (ab219910) is a cell- impermeable reagent designed to bind to phosphatidylserine (PS) residues exposed on the outer cell surface of cells with a flow cytometer or fluorescence microscopy at Ex/Em = 690/716 nm.


    We recommend using an impermeable nuclear stain such as Propidium Iodide (ab14083) together with Annexin V-iFluor 700 Detection Reagent to discriminate necrotic and dead cells: plasma membrane is disrupted in these cells and therefore the Annexin V reagent will bind to PS found in the interior of cells.

  • Notes

    Apoptosis is a regulated process of cell death that occurs during embryonic development as well as maintenance of tissue homeostasis. Inappropriately regulated apoptosis is implicated in different disease states, such as neurodegeneration disease and cancer. The apoptosis program is characterized by morphologic features, including loss of plasma membrane asymmetry and attachment, condensation off the cytoplasm and nucleus, and compaction and fragmentation of the nuclear chromatin. Exposure of phosphatidylserine (PS) on the external surface of the cell membrane has been reported to occur in the early phases of apoptotic cell death, during which the cell membrane remains intact. In leukocyte apoptosis, PS on the outer surface of the cell marks the cell for recognition and phagocytosis by macrophages. The human vascular anticoagulant, annexin V, is a 35-36 kDa Ca2+ dependent phospholipid binding protein that has a high affinity for PS, and shows minimal binding to phosphatidylcholine and sphingomyelin. Changes in PS asymmetry, which can be analyzed by measuring annexin V binding to the cell membrane, are generally observed before morphological changes associated with apoptosis occurred and before membrane integrity is lost.

  • Platform

    Flow cytometer

Properties

Images

  • The detection of binding activity of ab219911
    The detection of binding activity of ab219911

    Annexin V-mFluor Violet450 Detection Reagent (ab219911), which has similar PS binding properties to Annexin V-iFluor 700 conjugate. Detection of phosphatidylserine (PS) exposure in Jurkat cells. Jurkat cells were left untreated (blue) or treated with 1 μM staurosporine (red) in a 37°C, 5% CO2 incubator for 5 hours. Cells were then incubated with Annexin V Detection Reagent for 30 minutes. The fluorescence intensity of Annexin V-mFluor 450 Detection Reagent was measured with a FACSCalibur (BD systems)flow cytometer using violet laser at Ex/Em = 405/450 nm.

    In live non-apoptotic cells, Annexin V-iFluor 700 conjugate detects innate apoptosis in non-induced cells, which is typically 2-6% of all cells. In apoptotic cells Annexin V-iFluor 700 conjugate binds to phosphatidylserine, which is located on the outer leaflet of the cell membrane, resulted in increased staining intensity.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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