Angiotensin I ELISA kit (ab136934)
Key features and details
- Sensitivity: 4.3 pg/ml
- Range: 3.9 pg/ml - 10000 pg/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Angiotensin I ELISA kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% AGT 20 163.6pg/ml 8.3% AGT 20 53.2pg/ml 7.3% AGT 20 25.1pg/ml 10% Inter-assay Sample n Mean SD CV% AGT 182.2pg/ml 7.9% AGT 48.1pg/ml 10% AGT 24.4pg/ml 7.6% -
Sample type
Serum, Plasma -
Assay type
Competitive -
Sensitivity
4.3 pg/ml -
Range
3.9 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Spike 77.3 38pg/ml - 3286pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Angiotensin I in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Angiotensin I in plasma and serum.
A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with a solution of LVV Hemorphin 7 conjugated to biotin, followed by a solution of polyclonal antibody to LVV Hemorphin 7. The plate is washed to remove unbound reagents. A solution of streptavidin-HRP conjugate is then added. After further incubation the excess reagents are washed away and TMB substrate is added, which is catalyzed by HRP to generate a yellow color. A stop solution changes this color from yellow to blue, and the intensity of this blue coloration is inversely proportional to the amount of LVV Hemorphin 7 captured in the plate.
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Notes
Analyte
Sequence Percent cross reactivities in the range of 0.1 pM - 500 nM Ang(1-12)
DRVYIHPFHLVI
0.025Ang I DRVYIHPFHL 100 Ang(1-9) DRVYIHPFH
0.000 Ang II DRVYIHPF 0.083 Ang(1-7) DRVYIHP 0.000 Ang A ARVYIHPF 0.182 Ang III RVYIHPF 0.000 Ang IV VYIHPF 0.000 LVV-hemorphin 7 LVVYPWTQRF 0.000 Bradykinin RPPGFSPFR 0.000 -
Platform
Microplate
Properties
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Storage instructions
Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml Angiotensin I Biotinylated Conjugate 1 vial Angiotensin I Polyclonal Antibody 1 vial Angiotensin I Standard 1 x 1µg Assay Buffer 16 1 x 30ml Goat anti-rabbit IgG Microplate (12 x 8 wells) 1 unit HRP- Streptavidin Conjugate 1 x 12.5µg Plate Sealer 2 units Stop Solution 2 1 x 10ml TMB Substrate 1 x 10ml -
Research areas
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Alternative names
- AGT
- Ang I
- Angiotensin I
- ANGT_HUMAN
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Database links
- Entrez Gene: 183 Human
- Omim: 106150 Human
- SwissProt: P01019 Human
- Unigene: 19383 Human
- Unigene: 19383 Human
Images
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Typical Standard Curve
Representative standard curve using ab136934.