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Human TAFI ELISA kit (ab272774)

Key features and details

  • Range: 2 ng/ml - 500 ng/ml
  • Sample type: Cit plasma, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Quantitative
  • Reacts with: Human

Overview

  • Product name

    Human TAFI ELISA kit

  • Detection method

    Colorimetric

  • Sample type

    Serum, EDTA Plasma, Cit plasma

  • Assay type

    Quantitative

  • Range

    2 ng/ml - 500 ng/ml

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human TAFI ELISA kit (ab272774) is intended for the quantitative determination of total TAFI antigen in human plasma and serum.


    Human TAFI will bind to the affinity purified capture antibody coated on the microtiter plate. TAFI and TAFIa will react with the antibody on the plate. After appropriate washing steps, horseradish peroxidase labeled polyclonal anti-human TAFI antibody binds to the captured protein. Excess primary antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human TAFI. Color development is directly proportional to the concentration of total TAFI in the samples.

  • Tested applications

    Suitable for: ELISAmore details

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Buffer 1 x 50ml
    Anti-Human TAFI HRP Antibody Lyophilized Vial 1 vial
    Human TAFI Standard Lyophilized Vial 1 vial
    TAFI ELISA Plate 1 unit
    TMB Substrate 1 x 10ml

  • Research areas

  • Relevance

    The thrombin-activatable fibrinolysis inhibitor (TAFI) is a hepatically secreted zymogen. It potently attenuates fibrinolysis by removing the fibrin C-terminal lysine and arginine residues that are important for the binding and activation of plasminogen. This molecule is also known as carboxypeptidase U (CPU). It is an unstable basic carboxypeptidase that circulates in human plasma in its proenzyme form.

  • Cellular localization

    Secreted

  • Alternative names

    • bA139H14.2
    • Carboxypeptidase B like protein
    • Carboxypeptidase B2
    • Carboxypeptidase B2 (plasma carboxypeptidase U)
    • Carboxypeptidase B2 plasma
    • Carboxypeptidase U
    • CarboxypeptidaseB2
    • CarboxypeptidaseU
    • CBP2
    • CP B2
    • CPB 2
    • CPB2
    • CPU
    • PCPB
    • Plasma carboxypeptidase B
    • Plasma carboxypeptidase B2
    • Plasma carboxypeptidase U
    • Thrombin activable fibrinolysis inhibitor
    • Thrombin activatable fibrinolysis inhibitor
    see all

  • Database links

    Images

    • Example data
      Example data

      A typical standard curve. Example only.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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