Ammonia Assay Kit (ab272538)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Other biological fluids, Urine
Overview
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Product name
Ammonia Assay Kit
See all Ammonia kits -
Detection method
Fluorescent -
Sample type
Urine, Other biological fluids -
Assay type
Quantitative -
Range
0.012 mM - 1 mM -
Product overview
Ammonia Assay Kit (ab272538) is simple, direct and automation-ready procedures for measuring NH3 are very desirable. This assay is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 360/450nm) is proportional to the ammonia concentration in the sample.
Fast and sensitive: Linear detection range of 0.012 - 1 mM ammonia.
Convenient and high-throughput: Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
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Tested applications
Suitable for: Functional Studiesmore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 200 tests Assay Buffer 1 x 20ml Reagent A 1 x 1ml Reagent B 1 x 1ml Standard (20mM Ammonium chloride) 1 x 400µl -
Research areas
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Relevance
Ammonia is an important source of nitrogen for living systems. Nitrogen is required for the synthesis of amino acids, which are the building blocks of protein. Ammonia is a metabolic product which is created through amino acid deamination. It plays an important role in both normal and abnormal animal physiology like normal animal acid/base balance.
Images
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Ammonia Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.