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Alexa Fluor® 647 Anti-Integrin beta 1 antibody [12G10] (ab202643)

Key features and details

  • Alexa Fluor® 647 Mouse monoclonal [12G10] to Integrin beta 1
  • Suitable for: ICC/IF, Flow Cyt
  • Knockout validated
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
  • Isotype: IgG1

Overview

  • Product name

    Alexa Fluor® 647 Anti-Integrin beta 1 antibody [12G10]
    See all Integrin beta 1 primary antibodies

  • Description

    Alexa Fluor® 647 Mouse monoclonal [12G10] to Integrin beta 1

  • Host species

    Mouse

  • Conjugation

    Alexa Fluor® 647. Ex: 652nm, Em: 668nm

  • Tested applications

    Suitable for: ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length native protein (purified) corresponding to Integrin beta 1.
    Database link: P05556

  • Positive control

    • ICC/IF: HeLa cells Flow cyt: Hap1-wt cells

  • General notes

    This monoclonal antibody to beta 1 integrin has been knockout validated in ICC/IF. The expected staining for beta 1 integrin was observed in wild type cells and the staining was not seen in knockout cells.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

Images

  • Flow Cytometry - Alexa Fluor® 647 Anti-Integrin beta 1 antibody [12G10] (ab202643)
    Flow Cytometry - Alexa Fluor® 647 Anti-Integrin beta 1 antibody [12G10] (ab202643)

    Overlay histogram showing HAP1 wildtype (green line) and HAP1-ITGB1 knockout cells (red line) stained with ab202643. Live HAP1 wildtype and HAP1-ITGB1 knockout cells were incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab202643, 0.1ug/ml) for 30 min at 4°C.

    A mouse IgG1 isotype control antibody  was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-ITGB1 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5,000 events were collected using a 40 mW Red laser (647nm) and 670/14 bandpass filter.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Integrin beta 1 antibody [12G10] (ab202643)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Integrin beta 1 antibody [12G10] (ab202643)

    ab202643 staining Integrin β1 (shown in red) in wild-type HAP1 cells (top panel) and Integrin β1 knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3 M glycine in 0.1% PBS-Tween for 1 h. The cells were then incubated with ab202643 at 1/200 dilution (shown in red) and ab6046 (beta tubulin shown in green) at 1 μg/ml concentration overnight at +4°C, followed by a further incubation at room temperature for 1 h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Integrin beta 1 antibody [12G10] (ab202643)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Integrin beta 1 antibody [12G10] (ab202643)

    ab202643 staining Integrin beta 1 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab202643 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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