Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab197491)
![Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab197491)](https://www.abcam.com/ps/products/197/ab197491/Images/ab197491-239503-anti-cox-iv-antibody-mabcam33985-mitochondrial-marker-alexa-fluor-647-immunofluorescence.jpg "Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab197491)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [mAbcam33985] to COX IV - Mitochondrial Marker
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker
See all COX IV primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [mAbcam33985] to COX IV - Mitochondrial Marker -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: HeLa cells.
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
mAbcam33985 -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker (ab197491)
ab197491 staining COX IV in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab197491 at 1/1000 dilution (shown in red) and ab6046 (Rabbit polyclonal to beta Tubulin) at 1µg/ml. This was followed by an incubation at room temperature for 1h with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed, at 1µg/ml (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
