Astana Biomed Group, an authorized Abcam distributor in Central Asia

Alexa Fluor® 555 Anti-MEK1 + MEK2 antibody [EPR16667] (ab208076)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Alexa Fluor® 555 Rabbit monoclonal [EPR16667] to MEK1 + MEK2
  • Suitable for: ICC/IF
  • Reacts with: Human
  • Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm

Overview

  • Product name

    Alexa Fluor® 555 Anti-MEK1 + MEK2 antibody [EPR16667]
    See all MEK1 + MEK2 primary antibodies

  • Description

    Alexa Fluor® 555 Rabbit monoclonal [EPR16667] to MEK1 + MEK2

  • Host species

    Rabbit

  • Conjugation

    Alexa Fluor® 555. Ex: 555nm, Em: 565nm

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment within Human MEK1 + MEK2 aa 1 to the C-terminus. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. Also SwissProt ID: P36507
    Database link: Q02750

  • Positive control

    • ICC/IF: A431 cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16667

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-MEK1 + MEK2 antibody [EPR16667] (ab208076)
    Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-MEK1 + MEK2 antibody [EPR16667] (ab208076)

    ab208076 staining MEK1 + MEK2 in A431 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208076 at a 1/50 dilution (pseudocolored in green) and ab195884, Rat monoclonal to alpha Tubulin (Alexa Fluor® 647), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in A431 cells fixed with 4% formaldehyde (10 min).

  • Alexa Fluor® 555 Anti-MEK1 + MEK2 antibody [EPR16667] (ab208076)
    Alexa Fluor® 555 Anti-MEK1 + MEK2 antibody [EPR16667] (ab208076)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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