Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24118-2] to Angiotensinogen - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC, IP, Flow Cyt
  • Reacts with: Rat, Human

Overview

  • Product name

    Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free
    See all Angiotensinogen primary antibodies

  • Description

    Rabbit monoclonal [EPR24118-2] to Angiotensinogen - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human plasma, lung, liver and kidney tissue lysates; Rat plasma; Rat lung, kidney and liver tissue tissue lysate; HepG2 and Huh7 whole cell lysates. Flow cyt: HepG2 cells. ICC: HepG2 cells. IHC-P: Rat kidney tissue; Human placenta, liver, and kidney tissue. IP: HepG2 whole cell lysate.

  • General notes

    ab276137 is the carrier-free version of ab276132. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab276137 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)

    This data was developed using ab276132, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling Angiotensinogen with ab276132 at 1/500 (0.854 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on rat kidney (PMID: 28716988). The section was incubated with ab276132 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Immunocytochemistry - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    Immunocytochemistry - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)

    This data was developed using ab276132, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 100% Methanol permeabilized HepG2 cells labelling Angiotensinogen with ab276132 at 1/100 (4.27 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000  dilution (Green). Confocal image showing cytoplasmic staining in HepG2 cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Western blot - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    Western blot - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    All lanes : Anti-Angiotensinogen antibody [EPR24118-2] (ab276132) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate at 20 µg
    Lane 2 : Huh7 (human hepatocellar carcinoma epithelial cell), whole cell lysate
    Lane 3 : Human lu tissue lysate
    Lane 4 : Human liver tissue lysate
    Lane 5 : Human kidney tissue lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 48-70 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab276132, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Angiotensinogen undergoes glycosylation. The molecular weight observed is consistent with what has been described in literature(PMID: 9694881).

    Lanes 1 & 2 of the blot were developed using a higher sensitivity ECL substrate.

    Exposure times: Lane 1, 2: 59 seconds; Lane 3: 3.25 seconds;Lane 4, 5: 15 seconds

  • Flow Cytometry - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    Flow Cytometry - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)

    This data was developed using ab276132, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling Angiotensinogen with ab276132 at 1/500 dilution (0.1ug)(Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    Immunoprecipitation - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)

    This data was developed using ab276132, the same antibody clone in a different buffer formulation.

    Angiotensinogen was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate with ab276132 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab276132 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate 10 ug

    Lane 2: ab276132 IP in HepG2 whole cell lysate

    Lane 3:  Rabbit monoclonal IgG (ab172730) instead of ab276132 in HepG2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 23 seconds.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)

    This data was developed using ab276132, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Angiotensinogen with ab276132 at 1/500 (0.854 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human placenta (PMID: 21215447). The section was incubated with ab276132 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Western blot - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    Western blot - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    All lanes : Anti-Angiotensinogen antibody [EPR24118-2] (ab276132) at 1/1000 dilution

    Lane 1 : Rat lung tissue lysate
    Lane 2 : Rat liver tissue lysate
    Lane 3 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 48-70 kDa why is the actual band size different from the predicted?



    This data was developed using ab276132, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Angiotensinogen undergoes glycosylation. The molecular weight observed is consistent with what have been described in literature(PMID: 9694881).

    This blot was developed using a higher sensitivity ECL substrate.

    Exposure time: 127 seconds.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)

    This data was developed using ab276132, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Angiotensinogen with ab276132 at 1/500 (0.854 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human kidney. The section was incubated with ab276132 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Western blot - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    Western blot - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    All lanes : Anti-Angiotensinogen antibody [EPR24118-2] (ab276132) at 1/1000 dilution

    Lane 1 : Human plasma
    Lane 2 : Rat plasma

    Lysates/proteins at 20 µl per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/100000 dilution

    Predicted band size: 53 kDa
    Observed band size: 48-70 kDa why is the actual band size different from the predicted?



    This data was developed using ab276132, the same antibody clone in a different buffer formulation. 

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Angiotensinogen undergoes glycosylation. The molecular weight observed is consistent with what have been described in literature(PMID: 9694881).

    Exposure times: 

    Lane 1: 15 seconds
    Lane 2: 3.25 seconds

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)

    This data was developed using ab276132, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Angiotensinogen with ab276132 at 1/500 (0.854 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on human liver. The section was incubated with ab276132 for 30 minutes at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.

  • Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)
    Anti-Angiotensinogen antibody [EPR24118-2] - BSA and Azide free (ab276137)

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