Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)
![Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)](https://www.abcam.com/ps/products/208/ab208514/Images/ab208514-265142-anti-cd3d-antibody-ep4426-alexa-fluor-555-immunofluorescence.jpg "Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Alexa Fluor® 555 Rabbit monoclonal [EP4426] to CD3D
- Suitable for: ICC/IF
- Reacts with: Human
- Conjugation: Alexa Fluor® 555. Ex: 555nm, Em: 565nm
Overview
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Product name
Alexa Fluor® 555 Anti-CD3D antibody [EP4426]
See all CD3D primary antibodies -
Description
Alexa Fluor® 555 Rabbit monoclonal [EP4426] to CD3D -
Host species
Rabbit -
Conjugation
Alexa Fluor® 555. Ex: 555nm, Em: 565nm -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC/IF: Jurkat cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
EP4426 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)
ab208514 staining CD3 in Jurkat cells. The cells were fixed with 4% formaldehyde (10 min) and then incubated in 1%BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated overnight at +4°C with ab208514 at 1/100 dilution (pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in Jurkat cells fixed with 80% methanol (5 min).
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![Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)](https://www.abcam.com/ps/products/208/ab208514/Images/ab208514-2-benefits-of-recombinant-antibodies.png)
Alexa Fluor® 555 Anti-CD3D antibody [EP4426] (ab208514)
