Alexa Fluor® 488 Anti-p53 antibody [9D3DE3] (ab156030)
![Alexa Fluor® 488 Anti-p53 antibody [9D3DE3] (ab156030)](https://www.abcam.com/ps/products/156/ab156030/Images/ab156030-273069-anti-p53-antibody-9d3de3-alexa-fluor-488-flow-cytometry.jpg "Alexa Fluor® 488 Anti-p53 antibody [9D3DE3] (ab156030)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [9D3DE3] to p53
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2a
Overview
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Product name
Alexa Fluor® 488 Anti-p53 antibody [9D3DE3]
See all p53 primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [9D3DE3] to p53 -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Synthetic peptide corresponding to Human p53 aa 1-100 (N terminal).
Database link: P04637 -
Positive control
- Flow Cyt: HEK293 cells.
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General notes
This conjugated antibody has been KO validated based on the results obtained with the unconjugated clone: Anti-p53 antibody [9D3DE3] (ab154036).
Product was previously marketed under the MitoSciences sub-brand.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, 68% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Near homogeneity as judged by SDS-PAGE. ab156030 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
9D3DE3 -
Isotype
IgG2a -
Research areas
Images
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Flow Cytometry - Alexa Fluor® 488 Anti-p53 antibody [9D3DE3] (ab156030)
Overlay histogram showing HEK293 cells stained with ab156030 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab156030, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Mouse IgG2a - Alexa Fluor® 488 (ab171464) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
This antibody gave a positive signal in HEK293 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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![Flow Cytometry - Alexa Fluor® 488 Anti-p53 antibody [9D3DE3] (ab156030)](https://www.abcam.com/ps/products/156/ab156030/Images/ab156030-163379-anti-p53-antibody-9d3de3-alexa-fluor-488-flow-cytometry.jpg)
Flow Cytometry - Alexa Fluor® 488 Anti-p53 antibody [9D3DE3] (ab156030)Flow cytometric analysis of HEK293 cells (fixed and permeabilized with methanol) labeling p53 with ab156030 at 1 µg/ml (red) or a negative (nonreactive Alexa488-conjugated control antibody (green). Unstained cells (black) were also analyzed. 1% BSA in PBS was used as the blocking reagent for all the blocking steps.
