Alexa Fluor® 488 Anti-CD41 antibody [M148] (ab233053)
![Alexa Fluor® 488 Anti-CD41 antibody [M148] (ab233053)](https://www.abcam.com/ps/products/233/ab233053/Images/ab233053-351185-antiCD41ab233053Flow-CytometryHumanHEL-9217-Cells.jpg "Alexa Fluor® 488 Anti-CD41 antibody [M148] (ab233053)")
Key features and details
- Alexa Fluor® 488 Mouse monoclonal [M148] to CD41
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: Alexa Fluor® 488. Ex: 495nm, Em: 519nm
- Isotype: IgG2a
Overview
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Product name
Alexa Fluor® 488 Anti-CD41 antibody [M148]
See all CD41 primary antibodies -
Description
Alexa Fluor® 488 Mouse monoclonal [M148] to CD41 -
Host species
Mouse -
Conjugation
Alexa Fluor® 488. Ex: 495nm, Em: 519nm -
Specificity
This antibody binds to some medulloblastoma and neuroblastoma cells and rhabdomyosarcoma and certain other solid tumours. No binding observed to marrow constituents other than platelets and megakaryocytes. -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
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Immunogen
Tissue, cells or virus corresponding to Human CD41. Homogenized human medulloblastoma tissue.
Database link: P08514 -
Positive control
- Flow cyt: HEL 92.1.7 cells
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
M148 -
Myeloma
P3x63-Ag8.653 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Alexa Fluor® 488 Anti-CD41 antibody [M148] (ab233053)
Overlay histogram showing left HEL92.1.7 positive cells and right negative Jurkat cells stained with ab233053 (red line). The cells were incubated in 1x PBS containing 10 % normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab233053) (1x106 in 100 μl at 1 μg/ml (1/500)) for 30 min on ice. Isotype control antibody (black line) was mouse IgG2a kappa; Alexa Fluor® 488 used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
