AKT 1/2/3 pS473 + AKT 1/2/3 Total ELISA Kit (ab253299)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1 µg/ml
- Range: 0.05 % - 3.12 %
- Sample type: Cell Lysate
- Assay type: Semi-quantitative
- Reacts with: Mouse, Human
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT 1 x 25ml 50X Cell Extraction Enhancer Solution 1 x 1ml 5X Cell Extraction Buffer PTR 1 x 12ml AKT 1/2/3 (pS473) Capture Antibody 1 x 1.5ml AKT 1/2/3 (pS473) Detector Antibody 1 x 1.5ml AKT 1/2/3 Total Capture Antibody 1 x 1.5ml AKT 1/2/3 Total Detector Antibody 1 x 1.5ml Lyophilized AKT Control Lysate 1 vial Plate Seal 1 unit SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Substrate 1 x 12ml -
Research areas
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Cellular localization
AKT 1/2/3 (Total): Cytoplasm. Nucleus. Cell membrane. -
Database links
- Entrez Gene: 10000 Human
- Entrez Gene: 1385 Human
- Entrez Gene: 207 Human
- Entrez Gene: 208 Human
- Entrez Gene: 208 Human
- Entrez Gene: 5594 Human
- Entrez Gene: 5595 Human
- Entrez Gene: 11651 Mouse
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Images
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Other - AKT 1/2/3 pS473 + AKT1/2/3 Total ELISA Kit (ab253299)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Typical cell lysate dilution seriesExample of AKT 1/2/3 (pS473) and AKT 1/2/3 Total control lysate dilution series in 1X Cell Extraction Buffer PTR.
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Example of a typical AKT 1/2/3 (PS473) and AKT1 Total recombinant protein standard curve.Example of a typical AKT 1/2/3 (PS473) and AKT1 Total recombinant protein standard curve. The proportion of total protein that is phosphorylated is unknown - data is indicative only. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Cell line analysis for AKT 1/2/3 Total from preparations of 17,500 cells/well cell extracts.Data from triplicate measurements (mean +/- SD) are plotted.
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Induction of AKT 1/2/3 (pS473) phosphorylation in MCF-7 cells in response to insulin treatment, and levels of AKT 1/2/3 Total in the same samples.MCF-7 cells were cultured in 96-well tissue culture plates, serum-starved (2 hr) and treated (30 min) with a dose-range of insulin before cell lysis. Data from duplicate measurements of AKT 1/2/3 (pS473) are plotted and compared against AKT 1/2/3 Total protein levels.