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Signal Transduction Signaling Pathway Nuclear Signaling NFkB Pathway

Active human IKB alpha (phospho Y305) peptide (ab42717)

Active human IKB alpha (phospho Y305) peptide (ab42717)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Purity: > 90% SDS-PAGE
  • Active: Yes
  • Suitable for: Blocking, ELISA

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Description

  • Product name

    Active human IKB alpha (phospho Y305) peptide
    See all IKB alpha proteins and peptides
  • Biological activity

    The peptide is specifically recognized by IkBa (Tyr-305) phospho-specific antibody (ab24784) in ELISA, and has been shown to block the reactivity of ab24784 during Western blot. In addition, the peptide is recommended for use in blocking ab24784 reactivity in immunocytochemistry.
  • Purity

    > 90 % SDS-PAGE.

  • Animal free

    No
  • Nature

    Synthetic
    • Species

      Human
    • Modifications

      phospho Y305

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Preservative: 0.05% Sodium azide
    Constituent: PBS

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

Images

  • Blocking - Active human IKB alpha (phospho Y305) peptide (ab42717)
    Blocking - Active human IKB alpha (phospho Y305) peptide (ab42717)

    Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 min. Blots were probed with anti-IκBα (lane 1), anti-IκBα (Tyr-42) (lanes 2-5), or anti-IκBα (Tyr-305) (lanes 6-9). In some lanes, the antibodies were used in the absence (lane 2 & 6) or presence of ab42716, IκBα (Tyr-42) (lane 3 & 8) or ab42717, IκBα (Tyr-305) (lane 4 & 7) blocking peptides, or BSA conjugated to phospho-tyrosine (lane 5 & 9).

     

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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