Acidic Mammalian Chitinase Activity Kit (Fluorometric) (ab273291)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Fluorescent
- Platform: Microplate
- Sample type: Adherent cells, Cell Lysate, Suspension cells, Tissue, Tissue Lysate
- Sensitivity: 0.5 mU/ml
Overview
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Product name
Acidic Mammalian Chitinase Activity Kit (Fluorometric) -
Detection method
Fluorescent -
Sample type
Tissue, Adherent cells, Suspension cells, Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity (quantitative) -
Sensitivity
0.5 mU/ml -
Assay duration
Multiple steps standard assay -
Product overview
Acidic Mammalian Chitinase Activity Kit (Fluorometric) (ab273291) utilizes the ability of AMCase to cleave a synthetic substrate to release the free fluorophore which can be easily quantified (Ex/Em= 320/445 nm) and it uses a specific Lysis Buffer which distinguishes AMCase activity from CHIT1 activity. This kit provides a simple, specific, ultra-sensitive assay that can detect as low as 0.5 mU/ml of AMCase activity in a variety of biological samples.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 4-Methylumbelliferone Standard (5 mM) 1 x 35µl Acidic Mammalian Chitinase (Lyophilized) 1 vial AMCase Assay Buffer 1 x 25ml AMCase Lysis Buffer 1 x 18ml AMCase Substrate 1 x 25µl -
Research areas
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Function
Degrades chitin and chitotriose. May participate in the defense against nematodes, fungi and other pathogens. Plays a role in T-helper cell type 2 (Th2) immune response. Contributes to the response to IL-13 and inflammation in response to IL-13. Stimulates chemokine production by pulmonary epithelial cells. Protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1. Its function in the inflammatory response and in protecting cells against apoptosis is inhibited by allosamidin, suggesting that the function of this protein depends on carbohydrate binding. -
Tissue specificity
Detected in lung epithelial cells from asthma patients (at protein level). Highly expressed in stomach. Detected at lower levels in lung. -
Sequence similarities
Belongs to the glycosyl hydrolase 18 family. Chitinase class II subfamily.
Contains 1 chitin-binding type-2 domain. -
Cellular localization
Cytoplasm and Secreted. Secretion depends on EGFR activity. - Information by UniProt
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Alternative names
- Acidic mammalian chitinase
- Acidic mammalian chitinase [Precursor]
- AMCase
see all
Images
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Example standard curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
4-MU Standard Curve. Results from multiple experiments.
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AMCase Activity graphMeasurement of purified AMCase (2 ng) and CHIT1 (2 ng) activities. The kit can effectively discriminate AMCase activity from CHIT1 activity using our proprietary Lysis Buffer.
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Measurement of AMCase activity in different tissuesMeasurement of AMCase activity in Rat Liver (20 µg protein) and Mouse Lung (2.5 µg protein). All assays were performed following kit protocol.