2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) (ab174097)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Sample type: Adherent cells, Cell culture extracts, Suspension cells, Tissue Extracts
Overview
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Product name
2-Phosphoglycerate Assay Kit (Colorimetric/Fluorometric) -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture extracts, Adherent cells, Suspension cells, Tissue Extracts -
Assay type
Quantitative -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Abcam’s 2-Phosphoglycerate Assay kit (ab174097) is a sensitive, fast and easy-to-use kit. In this assay, 2PG(2-phosphoglycerate) is converted by Enzyme Mix to PEP (phosphoenolpyruvate), which is further converted to pyruvate. The pyruvate is oxidized to generate color (OD 570 nm) and fluorescence (Ex/Em = 535/587 nm). The colored product or fluorescence intensity is proportional to 2PG level. This assay kit can detect 2PG level below 20 pmol and can be used for a variety of sample types.
Visit our FAQs page for tips and troubleshooting.
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Notes
2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2PG is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). Measurement of intracellular 2PG levels is a useful tool for analyzing the glycolytic pathway and its relevance to cancer research.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests 2PG Assay Buffer 1 x 25ml 2PG Converter 1 vial 2PG Developer 1 vial 2PG Enzyme Mix (Lyophilized) 1 vial 2PG Probe (in DMSO) 1 x 0.2ml 2PG Standard 1 vial -
Research areas
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Relevance
2-phosphoglycerate (2PG) is an important intermediate in the glycolysis pathway. 2-Phosphoglycerate is converted by enolase to phosphoenolpyruvate (PEP) which is a key step from glucose to pyruvate. Aberrant glycolytic metabolism is a highly studied and potentially critical mechanism for ATP generation in cancer cells (The Warburg effect). -
Alternative names
- 2 Phosphoglycerate
- 2PG
Images
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2PG Standard Curve (Colorimetric)
Example of 2PG Standard curve obtained with 2-PG Assay Kit (ab174097) by colorimetric reading. Please not this data is example data only.
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2PG Standard Curve (Fluoroimetric)Example of 2PG Standard curve obtained with 2-PG Assay Kit (ab174097) by fluorometric reading. Please not this data is example data only.
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Measurement of 2PG levelsMeasurement of 2PG level in rat liver, heart and muscle lysate (200 µg protein each). Assays were performed according to kit protocol. This is example data only.