Astana Biomed Group, an authorized Abcam distributor in Central Asia

15-deoxy-delta12,14-PGJ2 ELISA Kit (ab133031)

Key features and details

  • Sensitivity: 36.8 pg/ml
  • Range: 195 pg/ml - 200000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Saliva, Urine
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Human, Pig, a wide range of other species

Overview

  • Product name

    15-deoxy-delta12,14-PGJ2 ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low 16 1136pg/ml 5.6%
    Medium 16 3062pg/ml 5.7%
    High 16 4394pg/ml 7.4%
    Inter-assay
    Sample n Mean SD CV%
    Low 8 1195pg/ml 15.7%
    Medium 8 4406pg/ml 13%
    High 8 7219pg/ml 14.5%

  • Sample type

    Cell culture supernatant, Saliva, Urine, Plasma

  • Assay type

    Competitive

  • Sensitivity

    36.8 pg/ml

  • Range

    195 pg/ml - 200000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 94.4 % - %
    Urine 113.9 % - %
    Plasma 105 % - %
    Tissue Culture Media 97.4 % - %

  • Assay time

    5h 0m

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human, Pig, a wide range of other species

  • Product overview

    Abcam’s 15-deoxy-delta12,14-PGJ2in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 15-deoxy-delta12,14-PGJ2 in tissue culture media, saliva, urine, and plasma from a wide range of species.


    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-15-deoxy-delta12,14-PGJ2 antigen and a polyclonal rabbit antibody specific to 15-deoxy-delta12,14-PGJ2. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 15-deoxy-delta12,14-PGJ2 captured in the plate.

  • Notes

    15-deoxy-Δ12,14-PGJ2 is one of the ultimate dehydration products of PGD2. PGD2 is formed from PGH2 which itself is synthesized from arachidonic acid by the enzyme prostaglandin synthase. In the aqueous solution PGD2 forms PGJ2. Albumin or other serum proteins present in the sample will convert PGD2 to the isomeric compound, Δ12-PGJ2. In males about 152 ng of Δ12-PGJ2 is excreted in urine per day, and in women about half this amount is excreted. 15-deoxy-Δ12,14-PGJ2 has been shown as an inducer of adipogenesis and an activator of the γ isoform of the peroxisome proliferation activated receptor (PPARγ).

    Cross Reactivity 

    Compound Cross Reactivity
    15-deoxy-Δ12,14-PGJ2 100%
    PGJ2 49.2%
    Δ12-PGJ2 5.99%
    PGD2 4.92%
    Arachidonic Acid 0.03%
    PGE2
    PGF2α
    9α,11β-PGF2α
    Thromboxane B2
    2-Arachidonoylglycerol
    Anandamide

  • Platform

    Microplate

Properties

Images

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab133031.

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