Antibodies, Proteins, Kits and Reagents for Life Science

403 418 ₸ Product size

100 µl 403 418 ₸ 10 µl 167 793 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2949] to CD9
Suitable for: WB, IP, IHC-P
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-CD9 antibody [EPR2949]
See all CD9 primary antibodies
Description

Rabbit monoclonal [EPR2949] to CD9
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Rat Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human CD9 aa 200 to the C-terminus. The exact sequence is proprietary.
Database link: P21926
Positive control
- WB: HeLa, HuT-78, MCF7 and U87-MG cell lysates. Mouse heart and kidney lysate; Rat brain lysate. IHC-P: Human papillary carcinoma , astrocytoma, brain, kidney and tonsil tissue; Rat spleen tissue. IP: HeLa whole cell lysate (ab150035).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR2949
Isotype

IgG
Research areas

Western blot - Anti-CD9 antibody [EPR2949] (ab92726)

All lanes : Anti-CD9 antibody [EPR2949] (ab92726) at 1/1000 dilution

Lane 1 : Wild-type A549 whole cell lysate
Lane 2 : CD9 knockout A549 whole cell lysate
Lane 3 : MCF7 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 25 kDa

Lanes 1 - 3: Merged signal (red and green). Green - ab92726 observed at 22 kDa. Red - loading control, ab9484, observed at 37 kDa.

ab92726 was shown to recognize CD9 in wild-type A549 cells as signal was lost at the expected MW in CD9 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD9 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. Ab92726 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)

Immunohistochemical staining of paraffin embedded rat spleen with purified ab92726 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunoprecipitation - Anti-CD9 antibody [EPR2949] (ab92726)

ab92726 (purified) at 1/20 immunoprecipitating CD9 in 10 μg HeLa (Lanes 1 and 2, observed at 24 kDa). Lane 3 - PBS. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST
Dilution buffer and concentration: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)

Formalin-fixed, paraffin-embedded Papillary carcinoma of thyroid gland tissue stained for CD9 using ab92726 at 1/500 dilution in immunohistochemical analysis.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-CD9 antibody [EPR2949] (ab92726)

All lanes : Anti-CD9 antibody [EPR2949] (ab92726) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell). Whole cell lysates with 5% NFDM/TBST
Lane 2 : Daudi (Human Burkitt's lymphoma lymphoblast). Whole cell lysates. (negative/low expression control) with 5% NFDM/TBST

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size: 25 kDa
Observed band size: 22 kDa
why is the actual band size different from the predicted?

Exposure time: 3 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)

Formalin-fixed, paraffin-embedded Normal kidney tissue stained for CD9 using ab92726 at 1/500 dilution in immunohistochemical analysis.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)

Formalin-fixed, paraffin-embedded Astrocytoma tissue stained for CD9 using ab92726 at 1/500 dilution in immunohistochemical analysis.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-CD9 antibody [EPR2949] (ab92726)

All lanes : Anti-CD9 antibody [EPR2949] (ab92726) at 1/10000 dilution (purified)

Lane 1 : HeLa cell lysate
Lane 2 : HuT-78 cell lysate
Lane 3 : U87-MG cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-CD9 antibody [EPR2949] (ab92726)

All lanes : Anti-CD9 antibody [EPR2949] (ab92726) at 1/2000 dilution (purified)

Lane 1 : mouse heart lysate
Lane 2 : mouse kidney lysate
Lane 3 : rat brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)

Formalin-fixed, paraffin-embedded Normal brain tissue stained for CD9 using ab92726 at 1/500 dilution in immunohistochemical analysis.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR2949] (ab92726)

Immunohistochemical staining of paraffin embedded human tonsil with purified ab92726 at a working dilution of 1/500. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Anti-CD9 antibody [EPR2949] (ab92726)

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