Z-VDVAD-FMK, caspase-2 inhibitor (ab142035)
Key features and details
- Irreversible caspase-2 inhibitor
- CAS Number:
- Soluble in DMSO to 20 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
Z-VDVAD-FMK, caspase-2 inhibitor -
Description
Irreversible caspase-2 inhibitor -
Biological description
Irreversible caspase-2 inhibitor. Inhibits apoptosis in multiple biological systems. -
Chemical structure
Properties
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Molecular weight
695.70 -
Molecular formula
C32H46FN5O11 -
Sequence
VDVAD (Modifications: N-terminal benzyloxycarbonyl; C-terminal FMK; Asp-2 = Asp(OMe); Asp-5 = Asp(OMe)) -
PubChem identifier
25108684 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO to 20 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one week. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CC(=O)OC)NC(=O)C(C(C)C)NC(=O)OCC1=CC=CC=C1 -
Source
Synthetic
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Research areas
Images
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Functional Studies - Z-VDVAD-FMK, caspase-2 inhibitor (ab142035)ab13847 staining active caspase 3 in SKNSH cells treated with Z-VDVAD-FMK (ab142035), by ICC/IF. Decrease in active caspase 3 expression correlates with increased concentration of Z-VDVAD-FMK, as described in literature.
The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab142035 (Z-VDVAD-FMK) in DMSO. After this incupation 10 µM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24 hours. The samples were then fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab13847 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with