Wound Healing Assay (ab242285)
Key features and details
- Assay type: Cell-based
- Detection method: Colorimetric/Fluorometric
- Platform: Microscope
- Sample type: Adherent cells
Overview
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Product name
Wound Healing Assay -
Detection method
Colorimetric/Fluorometric -
Sample type
Adherent cells -
Assay type
Cell-based -
Product overview
Wound Healing Assay Kit (ab242285) contains 2 x 24-well plates each containing 12 proprietary treated plastic inserts, or 1 x 24 well plate containing 6 proprietary treated plastic inserts. The inserts create a wound field with a defined gap of 0.9 mm for measuring the migratory and proliferation rates of cells. Migratory cells are able to extend protrusions and ultimately invade and close the wound field.
Cell proliferation and migration rates can be determined using manual fixing and microscopic imaging. A fixing solution is provided for stopping cells at specific time points. Cell stain and DAPI stain are also provided for viewing results with light and fluorescence microscopy.
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Platform
Microscope
Properties
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Storage instructions
Please refer to protocols. -
Components 24 tests 24-Well Wound Healing Assay Plate 1 unit Cell Stain Solution 1 x 4ml DAPI Fluorescence Stain (1000X) 1 x 10µl Fixation Solution 2 x 2ml -
Relevance
Wounded tissue initiates a complex and structured series of events in order to repair the damaged region. These events may include increased vascularization by angiogenic factors, an increase in cell proliferation and extracellular matrix deposition, and infiltration by inflammatory immune cells as part of the process to destroy necrotic tissue. The wound healing process begins as cells polarize toward the wound, initiate protrusion, migrate, and close the wound area. These processes reflect the behaviour of individual cells as well as the entire tissue complex.
Images
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Wound healing.
Wound healing.
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Assay Principle.Assay Principle.
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Wound Healing Assay Plate Assembly.Wound Healing Assay Plate Assembly.
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Example of Wound Field Surface Area.Example of Wound Field Surface Area.
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Percent Closure of MEF/STO Cells.STO cells were tested using ab242285. Cells were cultured 24 hours until a monolayer formed at which time the inserts were removed to begin the wound healing assay. Cells were monitored under phase contrast (not shown), DAPI labeling, and cell staining for determining percent closure (0, 50, 75, and 100%).