Whole Blood Polymorphonuclear Cell Isolation Kit (ab241001)
Key features and details
- Sample type: Whole Blood
Overview
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Product name
Whole Blood Polymorphonuclear Cell Isolation Kit -
Sample type
Whole Blood -
Product overview
Whole Blood Polymorphonuclear Cell Isolation Kit (ab241001) is unique in the market because it enables the user to isolate Polymorphonuclear cells (PMNs), assess viability of the obtained cell fractions and also determine purity with three simple, easy-to-use reagents. High yields, or 60-80% of total PMNs have been obtained yielding ≥ 5 x106 cells per mL of human whole blood. Purity of the cell fraction is assessed with giemsa stain (included). The kit’s use results in highly enriched (>95%) cell fraction of polymorphonuclear cells with less than 3% red blood cell counts. More than 99% of the cells are viable, as determined by the Viability Stain.
30 test refers to 30 mL isolation.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 30 tests Blunt-end needle 18 G; 1.5 in. (Sterile) 15 units Giemsa Stain 1 x 7ml PMN Density Gradient Media 2 x 20ml PMN Isolation Buffer (1X) 2 x 30ml Viability Stain 1 x 200µl -
Relevance
Polymorphonuclear cells (PMN) have a multilobed nucleus and include the following cells types: mast cells, neutrophils, eosinophils and basophils. Upon isolation, these cells can be used for migration assays and determination of cytokine/chemokine production in response to pharmacological compounds.
Images
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Isolation Steps
A. Illustration of conical tube held at 45 degrees while blood layered on top of PMN DGM. B.1. Layers of PMN DGM and whole blood prior to and after centrifugation showing the separation of layers in the conical tube. B.2 Illustrates separation of six layers (plasma, PBMCs, PMN DGM, PMNs, PMN DGM and RBCs) obtained after spinning whole blood with PMN DGM.
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Bright field and Fluorescent imageBright-Field image of hemocytometer showing total cells(left); image from Fluorescent microscope with Rhodamine/FITC filters of same ROI showing live (green) and dead (red) cells (middle); merge of two panels (right). D. Polymorphonuclear Cells stained with Giemsa stain indicating purity of cell suspension obtained.