Universal Lateral Flow Assay Kit (ab270537)
Overview
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Product name
Universal Lateral Flow Assay Kit -
Product overview
Universal Lateral Flow Assay Kit (ab270537) is designed to enable simple and rapid development of proof-of-concept sandwich lateral flow immunoassays. The kit includes everything needed for lateral flow assay development with any pair of capture and detection antibodies. It combines universal LFA strips, Lightning-Link® and Gold conjugation technologies, buffers and controls, which means there is no need to strip the capture antibody onto LFA strips using expensive equipment and complex procedures or conjugate the detection antibody to gold with passive absorption. The signal intensities can be qualitatively analyzed using the supplied scoring card or. For a quantitative detection, an LFA reader can be used.
This kit contains Gold conjugation kit (ab154873)
Benefits:
Adaptable to any pair of capture and detection antibodies
Quick and easy to use – conjugation protocols require only 30 seconds hands-on time
No need to strip or print capture antibodies
No passive absorption of detection antibodies
No specialised or costly equipment required
Results can be qualitatively and quantitatively analysed using the scoring card, or an LFA reader, respectively
This product is manufactured by Expedeon, an Abcam company. It was previously called Universal LFA Kit. Expedeon product code 4300-0100 is the same as the 100-test size of this product.
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Tested applications
Suitable for: LFAmore details
Properties
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Storage instructions
Please refer to protocols. -
Components 100 tests ab274297 - 40nm GOLD-Biotin, 10 OD 1 x 200µl ab274107 - 96-well clear low binding plates 2 units ab154873 - Gold Conjugation Kit (40nm, 20 OD) 1 kit ab274106 - Modifier reagent 1 x 200µl ab274296 - Quencher reagent 1 x 200µl Scoring card 1 unit ab274105 - Ulfa-Tag mix 3 x 100µg ab274103 - Universal LFA strips 1 x 100 tests ab274104 - Universal Running Buffer 2 x 1500µl
Images
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How this kit works
The capture antibody is conjugated to Lightning-Link® Ulfa-Tag, while the detection antibody is conjugated to 40 nm Gold, both of which require only 30 seconds to set up. The capture and detection antibody are diluted and incubated with the analyte and 40 nm Gold-Biotin and then run on Universal LFA strips. Universal LFA strips consist of a nitrocellulose membrane containing a ‘Test line’ (T-line) of immobilized anti-Ulfa-Tag antibody, that binds the Ulfa-Tag conjugated capture antibody which further binds the analyte in complex with the Gold-detection antibody. A red T-line appears when the analyte is present, and the line intensity varies depending on the analyte concentration. Universal LFA strips also contain a ‘Control-line’ (C-line) that exploit the extraordinarily high affinity of streptavidin for biotin, which shows that the test is valid, and an absorbent pad to promote and control the flow of sample through the membrane.
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Example dataExample of sandwich LFA assay for the detection of CRP in serum using the Universal Lateral Flow Assay kit: Ulfa-Tag and 40nm GOLD are used to conjugate the capture and detection antibody respectively. Results were analyzed using a Qiagen ESEQuant reader.