Abcam’s Thrombin cleavage kit (ab207000) efficiently removes tags from recombinant fusion proteins containing an accessible thrombin cleavage sequence.

Thrombin is a valuable biochemical tool due to its high proteolytic specificity. A thrombin cleavage site (e.g., Leu-Val-Pro-Arg-ll-Gly-Ser; where ll denotes the cleavage site) is widely incorporated within the linker region of fusion or affinity tagged recombinant proteins. After successful cleavage with thrombin, affinity tags or fused proteins can be separated from the target protein. Abcam's Thrombin cleavage kit provides an easy approach to test and optimize cleavage conditions of a target fusion or affinity-tagged protein containing a thrombin-specific cleavage site. The kit contains active thrombin enzyme sufficient to cleave up to 5 mg of the target protein. A 6x His-tagged protein containing the thrombin cleavage site is included as a cleavage control protein. Following cleavage of the target protein, thrombin can be removed by passing the reaction mix through a Heparin Sepharose^® column.