Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)
![Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)](https://www.abcam.com/ps/products/250/ab250284/Images/ab250284-4-anti-tmem161a-antibody-epr14369-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.JPG "Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14369] to TMEM161A - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Human
Overview
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Product name
Anti-TMEM161A antibody [EPR14369] - BSA and Azide free
See all TMEM161A primary antibodies -
Description
Rabbit monoclonal [EPR14369] to TMEM161A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250284 is the carrier-free version of ab180954. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250284 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14369 -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)This data was developed using ab180954, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling TMEM161A with ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)](https://www.abcam.com/ps/products/250/ab250284/Images/ab250284-3-anti-tmem161a-antibody-epr14369-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)This data was developed using ab180954, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling TMEM161A with ab180954 at 1/100 dilution, followed by prediluted ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol. -
![Western blot - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)](https://www.abcam.com/ps/products/250/ab250284/Images/ab250284-402607-anti-tmem161a-antibody-epr14369-bsa-and-azide-free-western-blot.JPG)
Western blot - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)Anti-TMEM161A antibody [EPR14369] (ab180954) at 1/20000 dilution + Human fetal liver tissue lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?This data was developed using ab180954, the same antibody clone in a different buffer formulation.
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![Western blot - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)](https://www.abcam.com/ps/products/250/ab250284/Images/ab250284-398530-anti-tmem161a-antibody-epr14369-bsa-and-azide-free-western-blot.JPG)
Western blot - Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)All lanes : Anti-TMEM161A antibody [EPR14369] (ab180954) at 1/100000 dilution
Lane 1 : HT1080 cell lysate
Lane 2 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?This data was developed using ab180954, the same antibody clone in a different buffer formulation.
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![Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)](https://www.abcam.com/ps/products/250/ab250284/Images/ab250284-5-benefits-of-recombinant-antibodies.png)
Anti-TMEM161A antibody [EPR14369] - BSA and Azide free (ab250284)
