SWAP70 overexpression 293T lysate (whole cell) (ab94234)
Overview
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Product name
SWAP70 overexpression 293T lysate (whole cell) -
General notes
ab94234 is a 293T cell transfected lysate in which Human SWAP70 has been transiently over-expressed using a pCMV-SWAP70 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. Regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion. Tissue specificity: Expressed only in mature B-cells including those associated with mucosa-associated tissue and bronchus-associated tissue (PubMed:10681448). Widely expressed. Abundant in spleen, and fairly abundant in kidney, lung and liver. Also found in monocytes and macrophages (PubMed:12925760). Similarity: Contains 1 PH domain. Domain: The PH domain is essential for phosphatidylinositol 3,4,5-trisphosphate binding. PTM: Tyrosine-phosphorylated.
Images
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SDS-PAGE - SWAP70 overexpression 293T lysate (whole cell) (ab94234)ab94234 at 15µg/lane on an SDS-PAGE gel.
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Western blot - SWAP70 overexpression 293T lysate (whole cell) (ab94234)All lanes : Anti-SWAP70 antibody (ab89532) at 1/500 dilution
Lane 1 :SWAP70 overexpression 293T lysate (whole cell) (ab94234)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution