Astana Biomed Group, an authorized Abcam distributor in Central Asia

Substance P ELISA Kit (ab133029)

Key features and details

  • Sensitivity: 8.04 pg/ml
  • Range: 9.8 pg/ml - 10000 pg/ml
  • Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Human, Pig

Overview

  • Product name

    Substance P ELISA Kit

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Low conc 24 101pg/ml 6.7%
    Medium conc 24 1116pg/ml 4.5%
    High conc 24 6257pg/ml 5.2%
    Inter-assay
    Sample n Mean SD CV%
    Low conc 8 97pg/ml 4.2%
    Medium conc 8 1120pg/ml 7.3%
    High conc 8 6402pg/ml 7.3%

  • Sample type

    Cell culture supernatant, Saliva, Urine, Serum, Plasma

  • Assay type

    Competitive

  • Sensitivity

    8.04 pg/ml

  • Range

    9.8 pg/ml - 10000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Saliva 108.3 % - %
    Urine 105.8 % - %
    Serum 97.7 % - %
    Tissue Culture Media 81 % - %
    Hep Plasma 109.3 % - %

  • Assay duration

    Multiple steps standard assay

  • Species reactivity

    Reacts with: Human, Pig
    Predicted to work with: a wide range of other species

  • Product overview

    Abcam’s Substance P in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Substance P in biological fluids.

    A goat anti-rabbit IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Substance P antigen and a polyclonal rabbit antibody specific to Substance P. After incubation the excess reagents are washed away. pNpp substrate is added and after a short incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of Substance P captured in the plate.

  • Notes

    Substance P is a undecapeptide that displays a number of biological activities. The peptide was first discovered in 1931 by von Euler and Gaddum. They reported that extracts of equine brain and intestine contained a hypotensive and spasmogenic factor. The preparation, termed preparation P, was later found to be proteinaceous. The isolation and characterization of Substance P was carried out by Leeman’s group in 1970. The structure is shown below.

    Substance P:  H-Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2

    Substance P is synthesized in the ribosomes as a larger protein and then enzymatically converted into the active peptide. The peptide is widely distributed in the peripheral and central nervous systems of vertebrates, where it is thought to act as a neurotransmitter. In the peripheral system, Substance P is localized in the primary sensory neurons and neurons intrinsic to the gastrointestinal tract.

    Cross Reactivity

    Compound% Cross Reactivity
    Substance P100
    Substance P (3-11)

    85.9

    Physalaemin75.3
    Substance P (4-11)11.7
    Substance P (7-11)5.9
    α-Neurokinin0.8
    β-Neurokinin0.2
    Somatostatin
    Substance P (1-4)

  • Platform

    Microplate

Properties

Images

  • Competitive ELISA - Substance P ELISA Kit (ab133029)
    Competitive ELISA - Substance P ELISA Kit (ab133029)
    Substance P measured in human, rat and pig biological fluids; background signal subtracted (duplicates +/- SD).
  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab133029.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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