Streptokinase Activity Assay Kit (Colorimetric) (ab273313)
Key features and details
- Assay type: Enzyme activity (quantitative)
- Detection method: Colorimetric
- Platform: Microplate
- Sample type: Serum
- Sensitivity: 3 mU/well
Overview
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Product name
Streptokinase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Serum -
Assay type
Enzyme activity (quantitative) -
Sensitivity
3 mU/well -
Assay duration
Multiple steps standard assay -
Product overview
Streptokinase Activity Assay Kit (Colorimetric) (ab273313) can be used to evaluate Streptokinase produced in bulk culture and its effect on plasminogen activation in serum. The assay is simple, easy to perform and can be completed in 3 easy steps. The assay can detect as little as 3 mU of Streptokinase Activity in less than 60 mins.
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Tested applications
Suitable for: Othermore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Plasminogen (Lyophilized) 1 vial pNA Standard 1 x 20µl SK Assay Buffer 1 x 6.5ml SK Substrate 1 x 850µl SK Substrate Buffer 1 x 3.5ml Streptokinase (Lyophilized) 1 vial -
Research areas
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Relevance
Streptokinase is a metallo-enzyme produced by various strains of Streptococcus that can liquify blood clots by converting plasminogen to plasmin. Streptokinase is not a protease but it activates plasminogen by complexing with it. It is thought to prevent the formation of effective fibrin barriers around the site of infection, thereby contributing to the invasiveness of the cells. -
Alternative names
- ska
- skc
- skg
Images
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pNA Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
pNA Standard Curve with 0-50 nmoles pNA/well.
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Activity of StreptokinaseExample data.
The graph shows the increase in OD values generated when Streptokinase is added to serum (indicating the increased level of plasmin activated by Streptokinase) and compared to Serum Background (Streptokinase without serum). Streptokinase is the positive control.
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Specificity activity of StreptokinaseExample data.
Fold increase in absorbance with the addition of Streptokinase to serum-off-the-clot over Background (inset).