SRB Assay / Sulforhodamine B Assay Kit (ab235935)
Key features and details
- Assay type: Cell-based (quantitative)
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Adherent cells
Overview
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Product name
SRB Assay / Sulforhodamine B Assay Kit -
Detection method
Colorimetric -
Sample type
Adherent cells -
Assay type
Cell-based (quantitative) -
Product overview
SRB Assay / Sulforhodamine B Assay Kit ab235935 relies on the stoichiometric binding of SRB (Sulforhodamine B) dye to proteins under mild acidic conditions and its subsequent extraction under basic conditions. The amount of dye extracted is a proxy for cell mass and thus the number of cells in a sample.
The measurement of the number of cells in the assay allows its use for analysis of cell viability, cytotoxicity and cell proliferation.
In the assay, cultured cells are fixed on plates, stained with Sulforhodamine B, washed and dried, then the bound dye is solubilized and the absorbance of the dye in solution is measured at OD 565 nm.
Review our cell health assay guide to learn about our kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1000 tests 10X Solubilization Solution 1 x 22ml 20 mM Doxorubicin 1 x 100µl 20X Washing Solution 1 x 50ml Fixation Solution 1 x 55ml SRB Dye Solution 1 x 50ml
Images
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SRB assay / Sulforhodamine B assay performed with ab235935
Dose-response curve of HepG2 (a), MCF7 (b) and HEK-293 (c) cells, after exposure to doxorubicin for 72 hours, as determined using the Sulforhodamine B Cell Cytotoxicity Assay Kit (Colorimetric). Assays were performed according to the kit protocol in triplicate.