Sheep IgG ELISA Kit (ab190546)
Key features and details
- Sensitivity: 4.28 ng/ml
- Range: 15.625 ng/ml - 500 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Sheep
Overview
-
Product name
Sheep IgG ELISA Kit
See all IgG kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% IgG Inter-assay Sample n Mean SD CV% IgG -
Sample type
Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
4.28 ng/ml -
Range
15.625 ng/ml - 500 ng/ml -
Recovery
> 85 %
Sample specific recovery Sample type Average % Range Serum > 85 % - % -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Sheep -
Product overview
Abcam’s IgG Sheep ELISA Kit (ab190546) is a two-site enzyme linked immunoassay (ELISA) for the measurement of IgG in sheep biological samples.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
-
Platform
Microplate
Properties
-
Components 1 x 96 tests 20X Diluent Concentrate 1 x 50ml 20X Wash Buffer Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml IgG Sheep Antibody coated microwells 1 unit IgG Sheep Calibrator (Lyophilized) 1 vial IgG Sheep HRP Conjugate 1 vial Stop Solution 1 x 12ml -
Research areas
-
Cellular localization
Secreted -
Alternative names
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
see all
Images
-
Standard Curve
Representative standard curve using ab190546 IgG Sheep ELISA Kit
