Sheep Anti-Mouse IgG H&L (beta-galactosidase) (ab136776)
Key features and details
- Sheep Anti-Mouse IgG H&L (beta-galactosidase)
- Conjugation: beta-galactosidase
- Host species: Sheep
- Isotype: IgG
- Suitable for: ICC, ELISA, IHC-P, IHC-Fr, WB
Overview
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Product name
Sheep Anti-Mouse IgG H&L (beta-galactosidase)
See all IgG secondary antibodies -
Host species
Sheep -
Target species
Mouse -
Specificity
Beta-galactosidase labelling provides good sensitivity and does not present endogenous activity in mammalian cells. It is well adapted to ELISA and produces no or very little background. It is more difficult to use in immunochemistry where its use can be justified only when the endogenous activities of other available labels produce significant problems. -
Tested applications
Suitable for: ICC, ELISA, IHC-P, IHC-Fr, WBmore details -
Immunogen
Highly purified whole immunoglobulin from mouse.
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Conjugation
beta-galactosidase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Constituents: 50% Glycerol, 50% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab136776 has been purified using a very stringent purification protocol where non-specific and specific purifications alternate (affinity chromatography). -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Preparation of Substrate Solutions: 0.1M phosphate buffer pH7, 1mM MgSO4, 0.2mM MnSO4, 2mM Mg titriplex. Add extemporaneously 7µl/ml beta-mercaptoethanol and 0.9mg/ml ortho-nitrophenyl-beta-galactopyranoside. Incubate for 30 minutes at 37°C. Stop the reaction with V/2 volume of 1M Na2CO3. -
Research areas