SETBP1 overexpression 293T lysate (whole cell) (ab94171)
Overview
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Product name
SETBP1 overexpression 293T lysate (whole cell) -
General notes
ab94171 is a 293T cell transfected lysate in which Human SETBP1 has been transiently over-expressed using a pCMV-SETBP1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Tissue specificity: Expressed in numerous tissues. Disease: Defects in SETBP1 are the cause of Schinzel-Giedion midface retraction syndrome (SGMFS) [MIM:269150]. It is a disorder characterized by severe mental retardation, distinctive facial features, and multiple congenital malformations including skeletal abnormalities, genitourinary and renal malformations, cardiac defects, as well as a higher-than-normal prevalence of tumors, notably neuroepithelial neoplasia. Similarity: Contains 3 A.T hook DNA-binding domains.
Images
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SDS-PAGE - SETBP1 overexpression 293T lysate (whole cell) (ab94171)ab94171 at 15μg/lane on an SDS-PAGE gel.
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Western blot - SETBP1 overexpression 293T lysate (whole cell) (ab94171)All lanes : Anti-SETBP1 antibody (ab68715) at 1/500 dilution
Lane 1 :SETBP1 overexpression 293T lysate (whole cell) (ab94171)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugate at 1/2500 dilution