SB 203580 hydrochloride, p38 MAPK inhibitor (ab120235)
Key features and details
- p38 MAPK inhibitor; water soluble
- CAS Number: 869185-85-3
- Purity: > 98%
- Soluble in water to 25 mM
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
SB 203580 hydrochloride, p38 MAPK inhibitor -
Description
p38 MAPK inhibitor; water soluble -
Biological description
Water soluble, cell-permeable p38 MAPK inhibitor. Shows selectivity over many other kinases but also shown to have some inhibitory activity against GAK, CK1 , RIP2 c-Raf and GSK3.
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Purity
> 98% -
CAS Number
869185-85-3 -
Chemical structure
Properties
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Chemical name
4-[4-(4-Fluorophenyl)-2-[4-(methylsulfinyl)phenyl]-1H-imidazol-5-yl]pyridine hydrochloride -
Molecular weight
413.90 -
Molecular formula
C21H16FN3OS.HCl -
PubChem identifier
16760644 -
Storage instructions
Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in water to 25 mM -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
Cl.CS(=O)c1ccc(cc1)c2nc(c(n2)c3ccncc3)c4ccc(F)cc4 -
Source
Synthetic
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Research areas
Images
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Functional Studies - SB 203580 hydrochloride, p38 MAPK inhibitor (ab120235)
MDA-MB-231 cells cells were incubated at 37°C for 24h with vehicle control (0 µM) and varied concentrations of SB 203580 hydrochloride (ab120235). Decreased expression of uPA receptor in MDA-MB-231 cells cells correlates with an increase in SB 203580 hydrochloride concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab103791 at 1 µg/ml and ab1801 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.