Renin Assay Kit (Fluorometric) (ab138875)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate
- Sensitivity: 10 ng/ml
Overview
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Product name
Renin Assay Kit (Fluorometric)
See all Renin kits -
Detection method
Fluorescent -
Sample type
Cell Lysate -
Assay type
Quantitative -
Sensitivity
10 ng/ml -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Renin Assay Kit (Fluorometric) (ab138875) provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a TF3/TQ3 FRET peptide. In the FRET peptide the fluorescence of TF3 is quenched by TQ3. Upon cleavage into two separate fragments by renin, the fluorescence of TF3 is recovered, the fluorescent signal can be easily monitored by a fluorescence microplate reader at Ex/Em = 540/590 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer 1 x 10ml Renin Red Substrate (100x) 1 x 50µl Renin Standard 1 x 25µl -
Research areas
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Function
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. -
Involvement in disease
Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - Information by UniProt
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Alternative names
- Angiotensin forming enzyme
- Angiotensin forming enzyme precursor
- Angiotensinogenase
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Images
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Sample Standard Curve for ReninRenin dose response was measured with ab138875 in a 96-well black solid plate using a fluorescence microplate reader. As low as 10 ng /mL Renin was detected with 60 minutes incubation in 37°C